Ontogeny of the stomach in yellow catfish (Pelteobagrus fulvidraco): detection and quantifictation of pepsinogen and H+/K+‐ATPase gene expression. Issue 1 (20th October 2011)

• Record Type: Journal Article
• Authors: Gao, C.; Yang, R. B.; Hu, W. B.; Wang, J.
• Is Part Of: Journal of animal physiology and animal nutrition. Volume 97:Issue 1(2013:Feb.)
• Page Start: 20
• Page End: 26
• Publication Date: 2011-10-20
• Subjects: Animal nutrition -- Periodicals; Feeds -- Periodicals; 636.085
• DOI: 10.1111/j.1439-0396.2011.01238.x
• Languages: English
• ISSNs: 0931-2439

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Summary</title> <p>Yellow catfish (<italic>Pelteobagrus fulvidraco</italic>) is an important commercial species with high aquaculture potential in China. To better understand the process of digestive functioning of gastric gland development during the larval from 1 dph (day post‐hatching) to 30 dph, real‐time PCR was used to detect and quantify the pepsinogen and H<sup>+</sup>/K<sup>+</sup>‐ATPase gene expression in <italic>P. fulvidraco</italic>. These data were also compared with the adult situation. The results showed that the expression of pepsinogen and H<sup>+</sup>/K<sup>+</sup>‐ATPase genes in <italic>P. fulvidraco</italic> larvae both started at 1 dph, though the expression level was very low until 3 dph. The quantification of pepsinogen gene expression increased significantly from 4 to 8 dph, increased fluctuantly from 8 to 23 dph and rose sharply from 23 to 30 dph. In comparison with adult fish, there were no significant differences with larvae at 5 and 23 dph. However, data of 10 and 30 dph larvae were obviously higher than those of adult group. H<sup>+</sup>/K<sup>+</sup>‐ATPase gene expression increased linearly from 1 to 30 dph. However, it was significantly lower than that of adult. The results show that <italic>P. fulvidraco</italic> larvae have an earlier functional stomach, though the function of the stomach is still not perfect. There is a gradual acidification environment within the stomach during the <italic>P. fulvidraco</italic> larvae development. Based on these results, we suggest that the weaning time for <italic>P. fulvidraco</italic> larvae would be much better after 23 dph.</p> </abstract>