Clonal diversity and genetic differentiation revealed by SSR markers in wild Vaccinium macrocarpon and Vaccinium oxycoccos. (6th November 2014)

• Record Type: Journal Article
• Authors: Zalapa, J.E.; Bougie, T.C.; Bougie, T.A.; Schlautman, B.J.; Wiesman, E.; Guzman, A.; Fajardo, D.A.; Steffan, S.; Smith, T.
• Is Part Of: Annals of applied biology. Volume 166:Number 2(2015)
• Page Start: 196
• Page End: 207
• Publication Date: 2014-11-06
• Subjects: Crop science -- Periodicals; Plants, Protection of -- Periodicals; Crops -- Ecology -- Periodicals; 630
• DOI: 10.1111/aab.12173
• Languages: English
• ISSNs: 0003-4746

Abstract

<abstract abstract-type="main" id="aab12173-abs-0001"> <title>Abstract</title> <p id="aab12173-para-0001">American cranberry (<italic>Vaccinium macrocarpon</italic>) is a perennial, woody plant species, native to North American bogs and wetlands. Cranberries represent one of the few agriculturally important native plants in which wild gene pools are still readily available within the undeveloped wetlands of the northern US and Canada. Earlier studies have reported low genetic variation in <italic>V. macrocarpon</italic> at the species and population level. However, in this study, we characterised 229 individuals of wild <italic>V. macrocarpon</italic> and <italic>V. oxycoccos</italic> (small cranberry) from Wisconsin and 22 accessions using microsatellite markers and observed substantial genetic variation and differentiation within and among populations and species. While <italic>V. macrocarpon</italic> was analysed using 108 alleles from 11 microsatellite loci revealing 42 unique genotypes, <italic>V. oxycoccos</italic> was analysed using 156 alleles from eight loci revealing 28 unique genotypes. There were a total of 182 alleles found in both species combined with 156 of those alleles present in <italic>V. oxycoccos</italic> and 84 alleles found in <italic>V. macrocarpon</italic>. All eight loci possessed species‐specific alleles with <italic>V. oxycoccos</italic> possessing 98 private alleles versus 26 private alleles found <italic>V. macrocarpon</italic>, and 58 alleles were found in common between both species. Our data will be valuable not only for future wild cranberry diversity and population genetics research, but for other cranberry breeding and genetics studies.</p> </abstract>