The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon. Issue 14 (18th July 2013)

• Record Type: Journal Article
• Authors: Voigt, Birgit; Schroeter, Rebecca; Jürgen, Britta; Albrecht, Dirk; Evers, Stefan; Bongaerts, Johannes; Maurer, Karl‐Heinz; Schweder, Thomas; Hecker, Michael
• Is Part Of: Proteomics. Volume 13:Issue 14(2013:Jul.)
• Page Start: 2140
• Page End: 2161
• Publication Date: 2013-07-18
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201200297
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The heat and ethanol stress response of <italic>Bacillus licheniformis</italic> DSM13 was analyzed at the transcriptional and/or translational level. During heat shock, regulons known to be heat‐induced in <italic>Bacillus subtilis</italic> 168 are upregulated in <italic>B. licheniformis</italic>, such as the HrcA, SigB, CtsR, and CssRS regulon. Upregulation of the SigY regulon and of genes controlled by other extracytoplasmic function (ECF) sigma factors indicates a cell‐wall stress triggered by the heat shock. Furthermore, tryptophan synthesis enzymes were upregulated in heat stressed cells as well as regulons involved in usage of alternative carbon and nitrogen sources. Ethanol stress led to an induction of the SigB, HrcA, and CtsR regulons. As indicated by the upregulation of a SigM‐dependent protein, ethanol also triggered a cell wall stress. To characterize the SigB regulon of <italic>B. licheniformis</italic>, we analyzed the heat stress response of a <italic>sigB</italic> mutant. It is shown that the <italic>B. licheniformis</italic> SigB regulon comprises additional genes, some of which do not exist in <italic>B. subtilis</italic>, such as <italic>BLi03885</italic>, encoding a hypothetical protein, the Na/solute symporter gene <italic>BLi02212</italic>, the arginase homolog‐encoding gene <italic>BLi00198</italic> and <italic>mcrA, </italic> encoding a protein with endonuclease activity.</p> </abstract>