Increased T-cell responses to Epstein–Barr virus with high viral load in patients with Epstein–Barr virus-positive diffuse large B-cell lymphoma. Issue 4 (April 2015)

• Record Type: Journal Article
• Authors: Morishima, Satoko; Nakamura, Shigeo; Yamamoto, Kazuhito; Miyauchi, Hidemasa; Kagami, Yoshitoyo; Kinoshita, Tomohiro; Onoda, Hiroshi; Yatabe, Yasushi; Ito, Masafumi; Miyamura, Kouichi; Nagai, Hirokazu; Moritani, Suzuko; Sugiura, Isamu; Tsushita, Keitaro; Mihara, Hidetsugu; Ohbayashi, Kaneyuki; Iba, Sachiko; Emi, Nobuhiko; Okamoto, Masataka; Iwata, Seiko; Kimura, Hiroshi; Kuzushima, Kiyotaka; Morishima, Yasuo
• Is Part Of: Leukemia & lymphoma. Volume 56:Issue 4(2015:Apr.)
• Page Start: 1072
• Page End: 1078
• Publication Date: 2015-04
• Subjects: Leukemia -- Periodicals; Lymphomas -- Periodicals; 616.99419
• DOI: 10.3109/10428194.2014.938326
• Languages: English
• ISSNs: 1042-8194

Abstract

<abstract> <title>Abstract</title> <p>The immunological status of patients with Epstein–Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (<italic>n</italic> = 13) were significantly higher than in healthy controls (HCs) (<italic>n</italic> = 16) after both <italic>ex vivo</italic> and <italic>in vitro</italic> stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after <italic>in vitro</italic> stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV− DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.</p> </abstract>