Effect of anionic macromolecules on intestinal permeability of furosemide. (February 2015)

• Record Type: Journal Article
• Authors: Valizadeh, Hadi; Fahimfar, Hadi; Ghanbarzadeh, Saeed; Islambulchilar, Ziba; Zakeri-Milani, Parvin
• Is Part Of: Drug development and industrial pharmacy. Volume 41:Number 2(2015:Feb.)
• Page Start: 190
• Page End: 193
• Publication Date: 2015-02
• Subjects: Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.3109/03639045.2013.851210
• Languages: English
• ISSNs: 0363-9045

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Furosemide is an anionic molecule and has very low absorption in gastro intestinal tract.</p> <p> <italic>Objective</italic>: The aim of this study was to investigate the effect of anionic macromolecules on the intestinal permeability of Furosemide.</p> <p> <italic>Materials and methods</italic>: The intestinal permeability of Furosemide was determined using single-pass intestinal perfusion technique in rats. Briefly a jejunal segment of ∼10 cm was isolated and cannulated in both ends for inlet and outlet solution. The perfusate was collected every 10 min and samples were analyzed using the RP-HPLC method. Test samples containing furosemide and two anionic macromolecules, sodium carboxy methyl cellulose and sodium alginate, at different concentrations were used.</p> <p> <italic>Results</italic>: The obtained data showed that existence of Sodium carboxy methyl cellulose significantly increased the <italic>P</italic><sub>eff</sub> values in all three investigated concentrations (<italic>p</italic> < 0.05) but sodium alginate only in concentrations <0.1% increased drug permeability.</p> <p> <italic>Discussion</italic>: It is concluded that the anionic macromolecules at specific concentrations could alter the permeability of anionic drugs across the biological membranes.</p> <p> <italic>Conclusions</italic>: Donnan phenomenon and chelating property of macromolecules could be attributed to the observed effect.</p> </abstract>