Characterization of Complementary Determinant Region 3δ in Human MutS Homologue 2‐Specific γδ T Cells. (February 2015)

• Record Type: Journal Article
• Authors: Chen, H.; Ji, X.; Cui, L.; Zhang, J.; He, W.
• Is Part Of: Scandinavian journal of immunology. Volume 81:Number 2(2015:Feb.)
• Page Start: 121
• Page End: 128
• Publication Date: 2015-02
• Subjects: Immunology -- Periodicals; 571.96
• DOI: 10.1111/sji.12256
• Languages: English
• ISSNs: 0300-9475

Abstract

<abstract abstract-type="main" id="sji12256-abs-0001"> <title>Abstract</title> <p> <italic>γδ</italic>T cells function as sentinels in early host responses to infections and malignancies. Previously, we found ectopically expressed human MutS homologue 2 (hMSH2), recognized by <italic>γδ</italic>T cells, triggered a <italic>γδ</italic>T cell‐mediated cytolysis to tumor cells. However, the characteristics of hMSH2‐specific <italic>γδ</italic> Τ cells are not fully understood. In this study, we investigated the complementary determinant region (CDR) 3<italic>δ</italic> diversity of hMSH2‐specific <italic>γδ</italic> T cells. We found that the CDR3<italic>δ</italic> sequences of hMSH2‐specific <italic>γδ</italic> T cells displayed limited diversity, while the length and germline gene usage showed no differences compared with whole CDR3<italic>δ</italic> immune repertoire. There are more hydrophilic amino acids in P/N insert of hMSH2‐specific <italic>γδ</italic> T cells including the more conserved amino acid at the position 97. Our results offer clues to understanding antigen recognition pattern of <italic>γδ</italic> T cells to stress‐induced hMSH2 of tumor cells and also the mechanism of <italic>γδ</italic>T cell‐mediated tumor immune surveillance.</p> </abstract>