Resistance and systemic dispersal of Xanthomonas fragariae in strawberry germplasm (Fragaria L.). (14th May 2014)

• Record Type: Journal Article
• Authors: Bestfleisch, M.; Richter, K.; Wensing, A.; Wünsche, J.N.; Hanke, M.‐V.; Höfer, M.; Schulte, E.; Flachowsky, H.
• Is Part Of: Plant pathology. Volume 64:Number 1(2015:Feb.)
• Page Start: 71
• Page End: 80
• Publication Date: 2014-05-14
• Subjects: Agricultural pests -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1111/ppa.12232
• Languages: English
• ISSNs: 0032-0862

Abstract

<abstract abstract-type="main" id="ppa12232-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The angular leaf spot disease caused by <italic>Xanthomonas fragariae</italic> is an important plant disease with major impact for the strawberry nursery industry. Currently there is no plant protection product available for controlling the disease effectively. Planting of resistant cultivars seems to be promising, but all commercially used cultivars are susceptible and no donor with a high level of resistance has yet been found. Therefore, a total of 145 genotypes from the Fruit Genebank Dresden (Germany) were evaluated for resistance to <italic>X. fragariae</italic> by artificial inoculation. Six genotypes were classified as partly resistant, out of which only two (US4808 and US4809) are octoploid. <italic>Fragaria vesca</italic> f. <italic>alba</italic>, <italic> Fragaria nilgerrensis</italic> 'Yunnan', <italic>F. vesca</italic> 'Illa Martin' and <italic>F. moschata</italic> 'Bauwens' were also classified as partially resistant, but they are only of limited use for breeding because of their variable ploidy level. Fully resistant genotypes could not be detected. The systemic dispersal of the bacteria in strawberry plants was investigated after inoculation of leaves with <italic>X. fragariae</italic> strain XF3.9.C and the GFP‐tagged strain XF3.9.C<sub>(pKAN)</sub>. The systemic spread was evaluated after 3, 7, 14 and 28 days post‐inoculation (dpi) by nested PCR and fluorescence microscopy. After 3 dpi, <italic>X. fragariae</italic> could be found in all tissues tested including the inoculated leaf, its petiole, the rhizome, the heart bud up to the youngest fully expanded leaf and its petiole. The systemic spread was also detectable in partially resistant genotypes.</p> </abstract>