Cardiac Troponin I as Compared to Troponin T for the Detection of Myocardial Damage in Horses. (January 2015)

• Record Type: Journal Article
• Authors: Van Der Vekens, N.; Decloedt, A.; Ven, S.; De Clercq, D.; van Loon, G.
• Is Part Of: Journal of veterinary internal medicine. Volume 29:Number 1(2015:Jan./Feb.)
• Page Start: 348
• Page End: 354
• Publication Date: 2015-01
• Subjects: Veterinary medicine -- Periodicals; 636.0896
• DOI: 10.1111/jvim.12530
• Languages: English
• ISSNs: 0891-6640

Abstract

<abstract abstract-type="main" id="jvim12530-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jvim12530-sec-0001" sec-type="section"> <title>Background</title> <p>Different cardiac troponin I (cTnI) assays give different results. Only 1 manufacturer has marketed troponin T (cTnT) assays. Therefore, cTnT often is preferred for detection of myocardial infarction in human patients. Studies of cTnT in horses are limited.</p> </sec> <sec id="jvim12530-sec-0002" sec-type="section"> <title>Objectives</title> <p>To compare a cTnI and a high‐sensitive cTnT assay (hs‐cTnT) in horses.</p> </sec> <sec id="jvim12530-sec-0003" sec-type="section"> <title>Animals</title> <p>Cardiac troponin I and cTnT were determined in 35 healthy horses (group 1), 23 horses suspected to have primary myocardial damage (group 2a), and 41 horses with secondary myocardial damage caused by structural heart disease (group 2b).</p> </sec> <sec id="jvim12530-sec-0004" sec-type="section"> <title>Methods</title> <p>All cTnI samples were analyzed at laboratory A (limit of detection [LOD]: 0.03 ng/mL), whereas cTnT samples were analyzed at 2 laboratories with the same hs‐cTnT assay (laboratory B, LOD: 10.0 pg/mL; laboratory C, LOD: 4.0 pg/mL).</p> </sec> <sec id="jvim12530-sec-0005" sec-type="section"> <title>Results</title> <p>The median cTnI concentration in group 2a (0.90 ng/mL; range, 0.03–58.27 ng/mL) was significantly higher (<italic>P</italic> < .001) than in group 1 (0.03 ng/mL; range, 0.03–0.09 ng/mL) or group 2b (0.05 ng/mL; range, 0.03–30.92 ng/mL), and the optimal cut‐off for detection of primary myocardial damage was 0.095 ng/mL (sensitivity: 90.5%, specificity: 100%). Using an LOD of 10.0 pg/mL for all cTnT samples, a cut‐off value of 10.5 pg/mL was found, but sensitivity was low (42.9%). When only samples analyzed at laboratory C (n = 58) were included, a cut‐off of 6.6 pg/mL was found (sensitivity: 81%, specificity: 100%).</p> </sec> <sec id="jvim12530-sec-0006" sec-type="section"> <title>Conclusions and Clinical Importance</title> <p>Despite large quantitative differences, cTnI and cTnT are both useful for detection of myocardial damage in horses.</p> </sec> </abstract>