Potential role of hematopoietic pre‐B‐cell leukemia transcription factor‐interacting protein in oral carcinogenesis. (24th July 2014)

• Record Type: Journal Article
• Authors: Okada, Seiji; Irié, Tarou; Tanaka, Junichi; Yasuhara, Rika; Yamamoto, Gou; Isobe, Tomohide; Hokazono, Chie; Tachikawa, Tetsuhiko; Kohno, Yohko; Mishima, Kenji
• Is Part Of: Journal of oral pathology & medicine. Volume 44:Number 2(2015:Feb.)
• Page Start: 115
• Page End: 125
• Publication Date: 2014-07-24
• Subjects: Dentistry -- Periodicals; Teeth -- Diseases -- Periodicals; 617
• DOI: 10.1111/jop.12210
• Languages: English
• ISSNs: 0904-2512

Abstract

<abstract abstract-type="main" id="jop12210-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jop12210-sec-0001" sec-type="section"> <title>Background</title> <p>Hematopoietic pre‐B‐cell leukemia transcription factor‐interacting protein (HPIP) is a corepressor of pre‐B‐cell leukemia homeobox (PBX) 1 and is known to play a role in hematopoiesis. Recently, HPIP was demonstrated to promote breast cancer cell proliferation and hepatocellular carcinoma growth. Moreover, it has been revealed that homeobox and PBX proteins, the expression of which is regulated by HPIP, play key roles in cancer of various organs, including oral squamous cell carcinoma (OSCC). Nevertheless, there has not been any study regarding the role of HPIP in OSCC. This study investigated the expression of HPIP in normal oral mucosa, epithelial precursor lesion (OEPL), and OSCC, and the functional roles of HPIP in OSCC cells and normal keratinocytes.</p> </sec> <sec id="jop12210-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Immunohistochemical analysis of HPIP, Ki‐67, and involucrin was performed in OSCC specimens, and the change in involucrin expression following RNA interference treatment against HPIP was examined by quantitative RT‐PCR and Western blot analysis in SCC9 and NHEK cells undergoing extracellular calcium‐induced differentiation. Matrigel transwell and cell proliferation assays for both cell lines transfected with HPIP siRNA were also conducted.</p> </sec> <sec id="jop12210-sec-0003" sec-type="section"> <title>Results</title> <p>HPIP expression increased in OEPL and OSCC specimens. <italic>In vitro</italic> analysis revealed that HPIP suppressed differentiation and proliferation of SCC9 cells and transwell migration of NHEK cells, while HPIP promoted invasion of SCC9 and proliferation of NHEK cells. However, HPIP has no significant effect on NHEK cell differentiation.</p> </sec> <sec id="jop12210-sec-0004" sec-type="section"> <title>Conclusion</title> <p>HPIP may play a critical role in oral carcinogenesis and is thus a potential target for anticancer therapy, with particular emphasis on its involvement in differentiation and migration/metastasis.</p> </sec> </abstract>