MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase MPK3 in both rice and Arabidopsis. (16th December 2014)

• Record Type: Journal Article
• Authors: Raghuram, Badmi; Sheikh, Arsheed H.; Rustagi, Yashika; Sinha, Alok K.
• Is Part Of: FEBS journal. Volume 282:Number 3(2015)
• Page Start: 521
• Page End: 536
• Publication Date: 2014-12-16
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.13159
• Languages: English
• ISSNs: 1742-464X

Abstract

<abstract abstract-type="main" id="febs13159-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>MicroRNA (miRNA) biogenesis requires AtDRB1 (double‐stranded RNA binding protein)/HYL1 (Hyponastic Leaves1) protein for processing and maturation of miRNA precursors. The AtDRB1/HYL1 protein associates with AtDCL1 (Dicer‐Like1) and accurately processes primary‐miRNAs (pri‐mRNAs) first to precursor‐miRNAs (pre‐miRNAs) and finally to mature miRNAs. The dephosphorylation of AtDRB1/HYL1 protein is very important for the precise processing of miRNA precursors. The monocot model crop plant <italic>Oryza sativa</italic> encodes four orthologues of AtDRB1/HYL1 protein, the only one encoded by <italic>Arabidopsis thaliana</italic>. The present study focuses on the functionality of the <italic>O. sativa</italic> DRBs as the orthologues of AtDRB1/HYL1 by using RNA binding assays and <italic>in planta</italic> protein–protein interaction analysis. Further, mitogen‐activated protein kinase MPK3 is established as the kinase phosphorylating DRB1 protein in both the model plants, <italic>O. sativa</italic> and Arabidopsis. MicroRNA microarray analysis in <italic>atmpk3</italic> and <italic>atmpk6</italic> mutants indicate the importance of AtMPK3 in maintaining the level of miRNAs in the plant.</p> </abstract>