Effect of storage temperature on the viability of human periodontal ligament fibroblasts. Issue 1 (19th September 2014)

• Record Type: Journal Article
• Authors: Chen, Fubo; Qi, Shengcai; Lu, Liyan; Xu, Yuanzhi
• Is Part Of: Dental traumatology. Volume 31:Issue 1(2015:Feb.)
• Page Start: 24
• Page End: 28
• Publication Date: 2014-09-19
• Subjects: Teeth -- Wounds and injuries -- Periodicals; Dentistry, Operative -- Periodicals; Traumatology -- Periodicals; Endodontics -- Periodicals; 617.6
• DOI: 10.1111/edt.12124
• Languages: English
• ISSNs: 1600-4469

Abstract

<abstract abstract-type="main" id="edt12124-abs-0001"> <title>Abstract</title> <sec id="edt12124-sec-0001" sec-type="section"> <title>Background</title> <p>The viability of periodontal ligament fibroblasts (PDLF) can affect the long‐term prognosis of replanted avulsed teeth. When immediate replantation of an avulsed tooth is not possible, the cells should be incubated in a physiological storage medium instantly to maintain their biological activity. The ability of different storage media to preserve PDLF viability has been previously evaluated. However, few studies have showed the effect of temperature on the viability of PDLF cultured with various storage media <italic>in vitro</italic>.</p> </sec> <sec id="edt12124-sec-0002" sec-type="section"> <title>Material and Methods</title> <p>This study was designed to measure PDLF activity by CCK‐8 assay to compare the effectiveness at 4, 22 (room temperature), and 37°C under various storage media.</p> </sec> <sec id="edt12124-sec-0003" sec-type="section"> <title>Results</title> <p>Statistical analysis demonstrated that tap water, saline, and saliva decreased cell viability as the storage temperature increased. But the temperature played only a minor role on cell viability when cells were incubated in Hank's balanced salt solution (HBSS), Dubelco's modified Eagle's medium (DMEM), or milk.</p> </sec> <sec id="edt12124-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Within the parameters of this study, it seems that room temperature is adequate for storing the avulsed teeth in HBSS, DMEM, or milk in the extra‐alveolar period.</p> </sec> </abstract>