Altered faecal and mucosal microbial composition in post‐infectious irritable bowel syndrome patients correlates with mucosal lymphocyte phenotypes and psychological distress. Issue 4 (18th December 2014)

• Record Type: Journal Article
• Authors: Sundin, J.; Rangel, I.; Fuentes, S.; Heikamp‐de Jong, I.; Hultgren‐Hörnquist, E.; de Vos, W. M.; Brummer, R. J.
• Is Part Of: Alimentary pharmacology & therapeutics. Volume 41:Issue 4(2015)
• Page Start: 342
• Page End: 351
• Publication Date: 2014-12-18
• Subjects: Digestive organs -- Diseases -- Treatment -- Periodicals; Digestive organs -- Effect of drugs on -- Periodicals; Gastrointestinal system -- Diseases -- Treatment -- Periodicals; Gastrointestinal system -- Effect of drugs on -- Periodicals; 615.73
• DOI: 10.1111/apt.13055
• Languages: English
• ISSNs: 0269-2813

Abstract

<abstract abstract-type="main" id="apt13055-abs-0001"> <title>Summary</title> <sec id="apt13055-sec-0001" sec-type="section"> <title>Background</title> <p>A subset of irritable bowel syndrome (IBS) patients, denoted post‐infectious IBS (PI‐IBS), develop symptoms after an enteric infection. Bacterial dysbiosis and mucosal inflammation have been proposed to be involved in the pathophysiology of this entity.</p> </sec> <sec id="apt13055-sec-0002" sec-type="section"> <title>Aim</title> <p>To characterise the mucosal and faecal microbiota in PI‐IBS, general IBS and healthy controls, and to investigate associations between the microbiota and the mucosal immune system.</p> </sec> <sec id="apt13055-sec-0003" sec-type="section"> <title>Methods</title> <p>Mucosal biopsies and faeces were collected from 13 PI‐IBS patients, 19 general IBS patients and 16 healthy controls. Global bacterial composition was determined by generating 16S rRNA amplicons that were examined by phylogenetic microarray hybridisation, principal component and redundancy analysis. We correlated previously reported lymphocyte proportions with the microbiota.</p> </sec> <sec id="apt13055-sec-0004" sec-type="section"> <title>Results</title> <p>Faecal microbiota composition of PI‐IBS patients differed significantly from both general IBS patients and healthy controls (<italic>P </italic><<italic> </italic>0.02). Both mucosal (<italic>P </italic><<italic> </italic>0.01) and faecal (<italic>P </italic>=<italic> </italic>0.05) microbial diversity were reduced in PI‐IBS compared to healthy controls.</p> <p>In the intraepithelial lymphocytes the previously published proportion of CD8<sup>+</sup> CD45RA<sup>+</sup> was negatively correlated with mucosal microbial diversity (<italic>P </italic><<italic> </italic>0.005). The previously published number of lamina propria lymphocytes was negatively correlated with mucosal microbial diversity (<italic>P </italic><<italic> </italic>0.05). Faecal microbial diversity was significantly negatively correlated with the Hospital Anxiety and Depression scale (<italic>P </italic><<italic> </italic>0.05).</p> </sec> <sec id="apt13055-sec-0005" sec-type="section"> <title>Conclusions</title> <p>We present data that distinguishes the intestinal microbiota of PI‐IBS patients from that of both general IBS patients and HC. The microbial composition is significantly associated with the HADs score and alterations in lymphocyte subsets proportions.</p> </sec> </abstract>