Heterogeneous nuclear ribonucleoprotein E1 regulates protein disulphide isomerase translation in oxidized low‐density lipoprotein‐activated endothelial cells. (25th November 2014)

• Record Type: Journal Article
• Authors: Meng, N.; Peng, N.; Huang, S.; Wang, S. Q.; Zhao, J.; Su, L.; Zhang, Y.; Zhang, S.; Zhao, B.; Miao, J.
• Is Part Of: Acta physiologica. Volume 213:Number 3(2015:Mar.)
• Page Start: 664
• Page End: 675
• Publication Date: 2014-11-25
• Subjects: Physiology -- Periodicals; Physiology -- Research -- Periodicals; 612
• DOI: 10.1111/apha.12422
• Languages: English
• ISSNs: 1748-1708

Abstract

<abstract abstract-type="main" id="apha12422-abs-0001"> <title>Abstract</title> <sec id="apha12422-sec-0001" sec-type="section"> <title>Aims</title> <p>Endothelium‐derived protein disulphide isomerase (PDI) is required for thrombus formation <italic>in vivo</italic>. But, how to control PDI overproduction in oxidized low‐density lipoprotein (oxLDL)‐activated vascular endothelial cells (VECs) is not well understood. In this study, we try to answer this question using our newly identified activator of mTOC1 3‐benzyl‐5‐((2‐nitrophenoxy) methyl)‐dihydrofuran‐2 (3H)‐one (3BDO) that has been shown to protect VECs.</p> </sec> <sec id="apha12422-sec-0002" sec-type="section"> <title>Methods</title> <p>First, we performed a proteomics analysis on the oxLDL‐activated vascular VECs in the presence or absence of 3BDO. Next, we constructed the heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1) mutants at Ser43 and used the RNA‐ChIP technique to investigate the relationship between hnRNP E1 and PDI production. Furthermore, we examined the effect of 3BDO on oxLDL‐altered phosphorylation of Akt1 and Akt2. Finally, we studied the effect of 3BDO on oxLDL‐altered PDI protein level in apolipoprotein E<sup>−/−</sup> mice with advanced atherosclerosis.</p> </sec> <sec id="apha12422-sec-0003" sec-type="section"> <title>Results</title> <p>In VECs, oxLDL‐increased PDI protein level, induced hnRNP E1 phosphorylation at Ser43, suppressed the binding of hnRNP E1 to <italic>PDI</italic> 5′UTR and induced the phosphorylation of Akt2 but not Akt1. All of these processes were blocked by 3BDO. Importantly, Ser43 mutant of hnRNP E1 inhibited the increase of PDI protein level and the decrease of the binding of hnRNP E1 and <italic>PDI</italic> 5′UTR induced by oxLDL. Furthermore, 3BDO suppressed oxLDL‐induced PDI protein increase in the serum and plaque endothelium of apolipoprotein E<sup>−/−</sup> mice.</p> </sec> <sec id="apha12422-sec-0004" sec-type="section"> <title>Conclusion</title> <p>hnRNP E1 is a new regulator of PDI translation in oxLDL‐activated VECs, and 3BDO is a powerful agent for controlling PDI overproduction.</p> </sec> </abstract>