Bacterial community of pond's water, sediment and in the guts of tilapia (Oreochromis niloticus) juveniles characterized by fluorescent in situ hybridization technique. (11th June 2013)

• Record Type: Journal Article
• Authors: Del'Duca, Alessandro; Cesar, Dionéia Evangelista; Abreu, Paulo César
• Is Part Of: Aquaculture research. Volume 46:Number 3(2015)
• Page Start: 707
• Page End: 715
• Publication Date: 2013-06-11
• Subjects: Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12218
• Languages: English
• ISSNs: 1355-557X

Abstract

<abstract abstract-type="main" id="are12218-abs-0001"> <title>Abstract</title> <p>Information about bacterial community structure and functioning in fish farming ponds remains scarce, mainly due to methodological difficulties in counting and identifying uncultured bacteria. The main objective of this study was to evaluate the degree of similarity between the bacterial community of the digestive tract of tilapia (<italic>Oreochromis niloticus</italic>) juveniles and that of the test pond's water and sediment, using the Fluorescent <italic>In Situ</italic> Hybridization (FISH) technique. Samples of water, sediment and gut content of 30 tilapia juveniles from a single nursery ground were collected in January 2010. Potentially probiotic and pathogenic bacteria of the species <italic>Bacillus</italic>, <italic> Lactobacillus brevis</italic>, <italic> Lactobacillus collinoides</italic>, <italic> Lactobacillus coryniformis</italic>, <italic> Lactobacillus farciminis</italic>, <italic> Vibrio</italic> and <italic>Pseudomonas fluorescens</italic> were found in different samples using specific fluorescent probes. The similarity between bacterial community environments and gastrointestinal tracts was determined using the Morisita‐Horn index. The fish guts presented higher abundances of <italic>Pseudomonas fluorescens</italic>, <italic> Bacillus</italic>, <italic> Lactobacillus brevis</italic> and <italic>Lactobacillus collinoides</italic>. The bacterial community composition of tilapia gastrointestinal tract was more similar to the water than the sediment of the pond. The results of this study showed that the FISH technique can be easily used for monitoring of probiotics and pathogen detection in aquaculture systems.</p> </abstract>