An in silico and chemical approach towards small protein production and application in phosphoproteomics1. Issue 25 (21st March 2015)

• Record Type: Journal Article
• Authors: Dias, Ana M. G. C.; Iranzo, Olga; Roque, Ana C. A.
• Is Part Of: RSC advances. Volume 5:Issue 25(2015)
• Page Start: 19743
• Page End: 19751
• Publication Date: 2015-03-21
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c4ra16934d
• Languages: English
• ISSNs: 2046-2069

Abstract

<abstract abstract-type="toc"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <graphic position="anchor" id="ga" xlink:href="ark:/27927/pgj416kz21" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" />This work demonstrates the advantages of peptide chemical synthesis as a robust method for the production and modification of small proteins, such as the human Pin1 WW domain. The protein was immobilized on a solid support and used to enrich phosphorylated species.</p> </abstract>