Development of Pasteurella multocida-loaded microparticles for hemorrhagic septicemia vaccine. (March 2015)

• Record Type: Journal Article
• Authors: Nimtrakul, Pataranapa; Atthi, Ratchanee; Limpeanchob, Nanteetip; Tiyaboonchai, Waree
• Is Part Of: Drug development and industrial pharmacy. Volume 41:Number 3(2015:Mar.)
• Page Start: 423
• Page End: 429
• Publication Date: 2015-03
• Subjects: Pharmaceutical chemistry -- Periodicals; Pharmaceutical industry -- Periodicals; Drug Industry -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615.05
• DOI: 10.3109/03639045.2013.873448
• Languages: English
• ISSNs: 0363-9045

Abstract

<abstract> <title>Abstract</title> <p>In this study, <italic>Pasteurella multocida-</italic>loaded alginate microparticles (MPs) for subcutaneous vaccination was developed by emulsification-cross-linking technique. Formulation parameter was varied as a ratio of polymer and bacterin. Optical microscopy revealed spherical particles with uniformly distribution. A mean particle size of approximately 6 µm has been successfully constructed using simple mixer and ultrasonic probe. The zeta potential of the MPs showed negatively charge of approximately −23 mV determined by Zeta Pals® analyzer. The entrapment efficiency and the <italic>in vitro</italic> bacterin released profile could be controlled by varying the amount of alginate. The high entrapment efficiency up to 69% was achieved with low concentration of alginate. The MPs possessed a slow bacterin release profile, up to 30 days. <italic>In vivo</italic> safety and potency tests were proved that the alginate MPs were safe and induced protective immunity in mice. In addition, after storage for 6 months at either 4 °C or room temperature, the protective immunity in mice was maintained.</p> </abstract>