Blood group B gene is barely expressed in in vitro erythroid culture of Bm‐derived CD34+ cells without an erythroid cell‐specific regulatory element. Issue 3 (18th December 2014)

• Record Type: Journal Article
• Authors: Sano, R.; Nogawa, M.; Nakajima, T.; Takahashi, Y.; Takahashi, K.; Kubo, R.; Kominato, Y.; Yokohama, A.; Tsukada, J.; Yamao, H.; Kishida, T.; Ogasawara, K.; Uchikawa, M.
• Is Part Of: Vox sanguinis. Volume 108:Issue 3(2015)
• Page Start: 302
• Page End: 309
• Publication Date: 2014-12-18
• Subjects: Blood -- Periodicals; Blood -- Transfusion -- Periodicals; Immunohematology -- Periodicals; Immunopathology -- Periodicals; 615.39
• DOI: 10.1111/vox.12220
• Languages: English
• ISSNs: 0042-9007

Abstract

<abstract abstract-type="main" id="vox12220-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="vox12220-sec-0001" sec-type="section"> <title>Background and Objectives</title> <p>Previously, a weak phenotype A<sub>m</sub> or B<sub>m</sub> was assumed to be caused by a reduction of A or B gene expression in bone marrow cells, but not in mucus‐secreting cells. However, <italic>ABO</italic> expression has not been examined in erythroid progenitor cells of A<sub>m</sub> or B<sub>m</sub> individuals.</p> </sec> <sec id="vox12220-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>We carried out <italic>in vitro</italic> erythroid differentiation of CD34<sup>+</sup> cells from peripheral blood of a B<sub>m</sub> individual harbouring a 3·0‐kb deletion including an erythroid cell‐specific regulatory element, named the +5·8‐kb site, in intron 1 of the human <italic>ABO</italic> blood group gene.</p> </sec> <sec id="vox12220-sec-0003" sec-type="section"> <title>Results</title> <p>During the <italic>in vitro</italic> differentiation of CD34<sup>+</sup> cells from this B<sub>m</sub> individual into erythroid cells, B‐antigens were not detectable on the cultured cells by flow cytometric analysis, and allele‐specific RT‐PCR consistently detected the transcripts from the <italic>O</italic> allele, but not from the <italic>B</italic> allele. Moreover, chromatin immunoprecipitation assay demonstrated that both RUNX1 and GATA‐2 or GATA‐1 were bound to the +5·8‐kb site in cultured erythroid cells expressing <italic>ABO</italic>.</p> </sec> <sec id="vox12220-sec-0004" sec-type="section"> <title>Conclusion</title> <p>It is likely that the +5·8‐kb site enhances transcription from the <italic>ABO</italic> promoter in erythroid cells through binding of RUNX1 and GATA‐2 or GATA‐1.</p> </sec> </abstract>