Elicitin‐like proteins Oli‐D1 and Oli‐D2 from Pythium oligandrum trigger hypersensitive response in Nicotiana benthamiana and induce resistance against Botrytis cinerea in tomato. (25th August 2014)

• Record Type: Journal Article
• Authors: Ouyang, Zhigang; Li, Xiaohui; Huang, Lei; Hong, Yongbo; Zhang, Yafen; Zhang, Huijuan; Li, Dayong; Song, Fengming
• Is Part Of: Molecular plant pathology. Volume 16:Number 3(2015:Apr.)
• Page Start: 238
• Page End: 250
• Publication Date: 2014-08-25
• Subjects: Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.12176
• Languages: English
• ISSNs: 1464-6722

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>The biocontrol agent <italic>Pythium oligandrum</italic> and its elicitin‐like proteins oligandrins have been shown to induce disease resistance in a range of plants. In the present study, the ability of two oligandrins, Oli‐D1 and Oli‐D2, to induce an immune response and the possible molecular mechanism regulating the defence responses in <italic>Nicotiana benthamiana</italic> and tomato were investigated. Infiltration of recombinant Oli‐D1 and Oli‐D2 proteins induced a typical immune response in <italic>N. benthamiana</italic> including the induction of a hypersensitive response (HR), accumulation of reactive oxygen species and production of autofluorescence. <italic>Agrobacterium</italic>‐mediated transient expression assays revealed that full‐length Oli‐D1 and Oli‐D2 were required for full HR‐inducing activity in <italic>N. benthamiana</italic>, and virus‐induced gene silencing‐mediated knockdown of some of the signalling regulatory genes demonstrated that NbSGT1 and NbNPR1 were required for Oli‐D1 and Oli‐D2 to induce HR in <italic>N. benthamiana</italic>. Subcellular localization analyses indicated that both Oli‐D1 and Oli‐D2 were targeted to the plasma membrane of <italic>N. benthamiana</italic>. When infiltrated or transiently expressed in leaves, Oli‐D1 and Oli‐D2 induced resistance against <italic>Botrytis cinerea</italic> in tomato and activated the expression of a set of genes involved in the jasmonic acid/ethylene (JA/ET)‐mediated signalling pathway. Our results demonstrate that Oli‐D1 and Oli‐D2 are effective elicitors capable of inducing immune responses in plants, probably through the JA/ET‐mediated signalling pathway, and that both Oli‐D1 and Oli‐D2 have potential for the development of bioactive formulae for crop disease control in practice.</p> </abstract>