Chemotactic Activity of Gestational Tissues Through Late Pregnancy, Term Labor, and RU486‐Induced Preterm Labor in Guinea Pigs. (20th October 2014)

• Record Type: Journal Article
• Authors: Gomez‐Lopez, Nardhy; Tong, Wing‐Chiu; Arenas‐Hernandez, Marcia; Tanaka, Satomi; Hajar, Omar; Olson, David M.; Taggart, Michael J.; Mitchell, Bryan F.
• Is Part Of: American journal of reproductive immunology. Volume 73:Number 4(2015:Apr.)
• Page Start: 341
• Page End: 352
• Publication Date: 2014-10-20
• Subjects: Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12333
• Languages: English
• ISSNs: 1046-7408

Abstract

<abstract abstract-type="main" id="aji12333-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="aji12333-sec-0001" sec-type="section"> <title>Problem</title> <p>Is increased leukocyte chemotactic activity (CA) from gestational tissues necessary for term or preterm labor in guinea pigs?</p> </sec> <sec id="aji12333-sec-0002" sec-type="section"> <title>Method of Study</title> <p>Tissue extracts were prepared from pregnant guinea pig decidua–myometrium, cervix, fetal membranes (amniochorion), and placenta during early third trimester (<italic>n</italic> = 8), term not in labor (TNL, <italic>n</italic> = 5), and term spontaneous labor (TL, <italic>n</italic> = 6), RU486‐induced preterm labor (PTL, <italic>n</italic> = 6), or controls (cPTL, <italic> n</italic> = 5). Leukocyte CA was assessed using a modified Boyden chamber assay. Extract chemokine and maternal progesterone concentrations were quantified by enzyme immunoassay.</p> </sec> <sec id="aji12333-sec-0003" sec-type="section"> <title>Results</title> <p>Only the extracts from amniochorion demonstrated increased CA through late gestation and labor. In contrast, CA was decreased in extracts from amniochorion and cervix from animals after RU486‐induced PTL. Maternal progesterone concentrations remained high in all groups.</p> </sec> <sec id="aji12333-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Leukocyte CA of intrauterine tissues is increased in term spontaneous labor. However, RU486‐induced preterm labor occurs in the absence of increased CA.</p> </sec> </abstract>