The Promiscuous and Synergic Molecular Interaction of Polyphenols in Bactericidal Activity: An Opportunity to Improve the Performance of Antibiotics?. (27th January 2015)

• Record Type: Journal Article
• Authors: Tomás‐Menor, Laura; Barrajón‐Catalán, Enrique; Segura‐Carretero, Antonio; Martí, Nuria; Saura, Domingo; Menéndez, Javier A.; Joven, Jorge; Micol, Vicente
• Is Part Of: Phytotherapy research. Volume 29:Number 3(2015:Mar.)
• Page Start: 466
• Page End: 473
• Publication Date: 2015-01-27
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5296
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Plant polyphenols are a potential source of new antimicrobial molecules against bacteria because most newly developed antimicrobial agents do not improve the clinical management of infectious diseases. The potential synergism between the major polyphenolic compounds present in a <italic>Cistus salviifolius</italic> extract, which was characterized by HPLC‐ESI‐MS/MS, was investigated by the isobole method and the fractional inhibitory concentration index determination. Pairwise combinations of selected flavonoids and ellagitannins present in <italic>C. salviifolius</italic> extract were assayed against the <italic>in vitro</italic> growth of <italic>Staphylococcus aureus</italic>. Some combinations revealed synergic effects, resulting in a reduction of the minimum inhibitory concentration required to inhibit 50% growth (MIC<sub>50</sub>) up to 20 times lower as compared with the individual compounds. Some of the combinations exhibited MIC<sub>50</sub> values close to drug potency level (0.5–1 µg/mL). Punicalagin and myricetin were the major contributors in the combinations. The proportion between the compounds in the synergic mixtures is crucial and may explain the superior antimicrobial activity displayed by this extract when compared with other botanical extracts. The rational optimization of these combinations could lead to the design of potent antimicrobial phytopharmaceuticals, which may improve the performance of current antibiotics, taking advantage of the multi‐targeted and synergic molecular interactions of selected polyphenols. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>