Application of CdTe/ZnSe Quantum Dots in In Vitro Imaging of Chicken Tissue and Embryo. (30th December 2014)

• Record Type: Journal Article
• Authors: Moulick, Amitava; Blazkova, Iva; Milosavljevic, Vedran; Fohlerova, Zdenka; Hubalek, Jaromir; Kopel, Pavel; Vaculovicova, Marketa; Adam, Vojtech; Kizek, Rene
• Is Part Of: Photochemistry and photobiology. Volume 91:Number 2(2015:Mar./Apr.)
• Page Start: 417
• Page End: 423
• Publication Date: 2014-12-30
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.12398
• Languages: English
• ISSNs: 0031-8655

Abstract

<abstract abstract-type="main" id="php12398-abs-0001"> <title>Abstract</title> <p>The present work is aimed to synthesize CdTe/ZnSe core/shell quantum dots (QDs) in an easy way and to explore the possibilities of its application in <italic>in vitro</italic> imaging of chicken tissue and embryo. The QDs were prepared using microwave irradiation with different temperatures, which is a very easy and less time‐consuming method. Subsequently, these QDs were characterized by spectrofluorimetry, Transmission Electron Microscopy, X‐ray fluorescence analysis and Dynamic Light Scattering measurement. A blueshifting of the emission was found when ZnSe was deposited on CdTe QDs. The QDs showed its fluorescence emission quantum yields up to 25%. They were applied into chicken embryos and breast muscle tissues to study their efficiency in <italic>in vitro</italic> imaging. All the QDs of different color were able to visualize in <italic>in vitro</italic> imaging. The highest fluorescence intensity was detected in the case of red QDs prepared at 100°C. The green and red QDs were possible to detect up to the depth of 3 and 4 mm of the tissue, respectively.</p> </abstract>