Impact of saline irrigation and topical corticosteroids on the postsurgical sinonasal microbiota. Issue 3 (29th December 2014)

• Record Type: Journal Article
• Authors: Liu, Cindy M.; Kohanski, Michael A.; Mendiola, Michelle; Soldanova, Katerina; Dwan, Michael G.; Lester, Richard; Nordstrom, Lora; Price, Lance B.; Lane, Andrew P.
• Is Part Of: International forum of allergy & rhinology. Volume 5:Issue 3(2015:Mar.)
• Page Start: 185
• Page End: 190
• Publication Date: 2014-12-29
• Subjects: 617.51005
• DOI: 10.1002/alr.21467
• Languages: English
• ISSNs: 2042-6976

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="alr21467-sec-0010" sec-type="section"> <title>Background</title> <p>Topical treatments with nasal saline irrigation, topical steroid sprays, or corticosteroid rinses can improve sinonasal symptoms in chronic rhinosinusitis (CRS). However, the impact of these therapies on commensals (<italic>Corynebacterium</italic>) and on biofilm pathogens associated with CRS (<italic>Staphylococcus aureus</italic> and <italic>Pseudomonas</italic>) is not well characterized.</p> </sec> <sec id="alr21467-sec-0020" sec-type="section"> <title>Methods</title> <p>Paired nasal and sinus swabs were collected endoscopically from 28 controls and 14 CRS patients with nasal polyposis (CRSwNP) who had not received systemic antibiotics or corticosteroids in the previous 8 weeks. Total DNA from swab eluents were extracted and analyzed by 16S rRNA gene‐based pyrosequencing. A total of 359, 077 reads were obtained and classified taxonomically. The association of use of topical therapies with sinonasal microbiota composition was assessed by factor/vector‐fitting. The proportional abundances of sinonasal bacteria between topical therapy users and nonusers were further compared by 2‐tailed Kolmogorov‐Smirnov test among controls and among CRSwNP participants.</p> </sec> <sec id="alr21467-sec-0030" sec-type="section"> <title>Results</title> <p>Nasal saline irrigation, with or without added budesonide, was not associated with significantly distinct sinonasal microbiota composition or significantly decreased <italic>Pseudomonas</italic> or <italic>S. aureus</italic> abundances among either controls or CRSwNP participants. <italic>Corynebacterium</italic> was slightly lower in controls that reported using saline irrigation than those who did not. No significant association was found between nasal saline irrigation and the proportional abundances of <italic>Pseudomonas</italic>, <italic>S. aureus</italic>, and <italic>Corynebacterium</italic> in CRSwNP participants. However, male CRSwNP patients were noted to have significantly higher <italic>Corynebacterium</italic> proportional abundances than their female counterparts. The use of topical steroid sprays was associated with a distinct microbiota in control subjects, characterized by higher proportional abundances of <italic>Dolosigranulum</italic> and <italic>Simonsiella</italic> and a lower proportional abundance of <italic>Campylobacter</italic>.</p> </sec> <sec id="alr21467-sec-0040" sec-type="section"> <title>Conclusion</title> <p>Nasal saline irrigation is not associated with a distinct alteration in the proportional abundance of commensal bacteria or biofilm‐forming pathogens in CRSwNP patients. However, use of topical intranasal corticosteroid sprays in control subjects is associated with a distinct sinonasal microbiota.</p> </sec> </abstract>