In vitro assessment of phytochemicals, antioxidant and DNA protective potential of wild edible fruit of Elaeagnus latifolia Linn. (11th July 2014)

• Record Type: Journal Article
• Authors: Panja, Sourav; Chaudhuri, Dipankar; Baban Ghate, Nikhil; Le Minh, Ha; Mandal, Nripendranath
• Is Part Of: Fruits. Volume 69:Number 4(2014)
• Page Start: 303
• Page End: 314
• Publication Date: 2014-07-11
• Subjects: Fruit-culture -- Periodicals; 634
• DOI: 10.1051/fruits/2014019
• Languages: English
• ISSNs: 0248-1294

Abstract

<abstract abstract-type="normal" xml:lang="en"> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <p> <bold>Introduction</bold>. <italic>Elaeagnus latifolia </italic>Linn. is a type of wild edible fruit found in northeast India, Thailand and also in Vietnam. Although the fruit is reported to be a source of vitamins, minerals, essential fatty acids and other bioactive compounds, only a few studies have been concerned with the antioxidant activity of this plant. <bold>Materials and methods</bold>. Our study revealed <italic>in vitro</italic> antioxidant and free radical scavenging activity of 70% methanolic extract of <italic>Elaeagnus latifolia</italic> Linn. (ELME). Various tests including identification and quantification of phytochemicals, total antioxidant activity, hydroxyl radical, superoxide radical, singlet oxygen, hypochlorous acid scavenging, reducing power and DNA protection assays were performed. <bold>Results and discussion</bold>. Among the tests, ELME scavenged superoxide radical [IC50 = (150.78 ± 4.2) μg×mL–1], hydroxyl radical [IC50 = (238.09 ± 11.63) μg×mL–1] and protected pUC18 DNA [P50 = (695.91 ± 15.84) μg×mL–1]; P50 signifies the concentration for 50% protection . The fruit is found to be a source of minute amounts of carbohydrates, ascorbic acid, tannins, phenolics and flavonoids. HPLC data showed that purpurin, tannic acid, quercetin, catechin, reserpine and rutin are present in ELME. <bold>Conclusion</bold>. Our results provide evidence that 70% methanol extract of <italic>E. latifolia</italic> Linn. acts as a promising antioxidant as well as DNA protector, which is partly due to the phenolic and flavonoid compounds present in it.</p> </abstract>