Evaluation of lactate detection using selective multiple quantum coherence in phantoms and brain tumours. (14th January 2015)

• Record Type: Journal Article
• Authors: Harris, L. M.; Tunariu, N.; Messiou, C.; Hughes, J.; Wallace, T.; DeSouza, N. M.; Leach, M. O.; Payne, G. S.
• Is Part Of: NMR in biomedicine. Volume 28:Number 3(2015:Mar.)
• Page Start: 338
• Page End: 343
• Publication Date: 2015-01-14
• Subjects: Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.3255
• Languages: English
• ISSNs: 0952-3480

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Lactate is a product of glucose metabolism. In tumour tissues, which exhibit enhanced glycolytic metabolism, lactate signals may be elevated, making lactate a potential useful tumour biomarker. Methods of lactate quantitation are complicated because of overlap between the lactate methyl doublet CH<sub>3</sub> resonance and a lipid resonance at 1.3 ppm. This study presents the use of a selective homonuclear multiple quantum coherence transfer sequence (SelMQC‐CSI), at 1.5 T, to better quantify lactate in the presence of lipids. Work performed on phantoms showed good lactate detection (49%) and lipid suppression (98%) efficiencies. To evaluate the method in the brain, the sequence was tested on a group of 23 patients with treated brain tumours, either glioma (<italic>N</italic> = 20) or secondary metastases in the brain (<italic>N</italic> = 3). Here it was proved to be of use in determining lactate concentrations <italic>in vivo</italic>. Lactate was clearly seen in SelMQC spectra of glioma, even in the presence of lipids, with high grade glioma (7.3 ± 1.9 mM, mean ± standard deviation) having higher concentrations than low grade glioma (1.9 ± 1.5 mM, <italic>p</italic> = 0.048). Lactate was not seen in secondary metastases in the brain. SelMQC‐CSI is shown to be a useful technique for measuring lactate in tumours whose signals are otherwise contaminated by lipid. © 2015 The Authors <italic>NMR in Biomedicine</italic> Published by John Wiley & Sons Ltd.</p> </abstract>