Laser‐induced shockwave paired with FRET: A method to study cell signaling. Issue 3 (13th January 2015)

• Record Type: Journal Article
• Authors: Gomez‐Godinez, Veronica; Preece, Daryl; Shi, Linda; Khatibzadeh, Nima; Rosales, Derrick; Pan, Yijia; Lei, Lie; Wang, Yingxiao; Berns, Michael W.
• Is Part Of: Microscopy research and technique. Volume 78:Issue 3(2015:Mar.)
• Page Start: 195
• Page End: 199
• Publication Date: 2015-01-13
• Subjects: Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22463
• Languages: English
• ISSNs: 1059-910X

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Cells within the body are subject to various forces; however, the details concerning the way in which cells respond to mechanical stimuli are not well understood. We demonstrate that laser‐induced shockwaves (LIS) combined with biosensors based on fluorescence resonance energy transfer (FRET) is a promising new approach to study biological processes in single live cells. As "proof‐of‐concept, " using a FRET biosensor, we show that in response to LIS, cells release intracellular calcium. With the parameters used, cells retain their morphology and remain viable. LIS combined with FRET permits observation of the cells immediate response to a sudden shear force. <italic>Microsc. Res. Tech. 78:195–199, 2015.</italic> © 2015 Wiley Periodicals, Inc.</p> </abstract>