Runx/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland. Issue 3 (15th December 2014)

• Record Type: Journal Article
• Authors: Islam, Md. Nurul; Itoh, Shinsuke; Yanagita, Takeshi; Sumiyoshi, Kumi; Hayano, Satoru; Kuremoto, Koh‐Ichi; Kurosaka, Hiroshi; Honjo, Tadashi; Kawanabe, Noriaki; Kamioka, Hiroshi; Sakai, Takayoshi; Ishimaru, Naozumi; Taniuchi, Ichiro; Yamashiro, Takashi; Trainor, Paul; Johnson, Randy
• Is Part Of: Developmental dynamics. Volume 244:Issue 3(2015:Mar.)
• Page Start: 488
• Page End: 496
• Publication Date: 2014-12-15
• Subjects: Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.24231
• Languages: English
• ISSNs: 1058-8388

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <underline>Background:</underline> The rodent salivary gland is not fully developed at birth and the cellular definitive differentiation takes place postnatally. However, little is known about its molecular mechanism. <bold><underline>Results:</underline></bold> Here we provide the loss‐of‐function genetic evidence that Runx signaling affects postnatal development of the submandibular gland (SMG). Core binding factor β (Cbfb) is a cotranscription factor which forms a heterodimer with Runx proteins. <italic>Cbfb</italic> was specifically expressed in the duct epithelium, specifically in the SMG. Epithelial Cbfb deficiency resulted in decrease in the size of the SMG and in the saliva secretion on postnatal day 35. The <italic>Cbfb</italic> mutant SMG specifically exhibited involution of the granular convoluted tubules (GCT), with a down‐regulated expression of its marker genes, such as <italic>Klk1</italic>, <italic>Ngf</italic>, and <italic>Egf</italic>. The induction of GCT is under the control of androgens, and the <italic>Cbfb</italic> mutant SMG demonstrated down‐regulated expression of <italic>Crisp3</italic>, an androgen‐dependent transcript. Because the circulating testosterone or tissue dihydrotestosterone levels were not affected in the <italic>Cbfb</italic> mutants, it appears that Runx/Cbfb signaling regulate androgen receptor pathway, but does not affect the circulating testosterone levels or the enzymatic conversion to DHT. <bold><underline>Conclusions:</underline></bold> Runx signaling is important in the postnatal development of androgen‐dependent GCT in the SMG. <italic>Developmental Dynamics 244:488–496, 2015</italic>. © 2014 Wiley Periodicals, Inc.</p> </abstract>