Electrospun Highly Ordered Mesoporous Silica–Carbon Composite Nanofibers for Rapid Extraction and Prefractionation of Endogenous Peptides. Issue 11 (30th January 2015)

• Record Type: Journal Article
• Authors: Zhu, Gang‐Tian; Chen, Xi; He, Xiao‐Mei; Wang, Han; Zhang, Zheng; Feng, Yu‐Qi
• Is Part Of: Chemistry. Volume 21:Issue 11(2015)
• Page Start: 4450
• Page End: 4456
• Publication Date: 2015-01-30
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201406237
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A simple method was developed for the preparation of ordered mesoporous silica–carbon composite nanofibers (OMSCFs). The OMSCFs exhibited high carbon content, continuously long fibrous properties, uniform accessible mesopores, and a large surface area. The OMSCFs were also found to have ion‐exchange capacity. On the basis of the size‐exclusion effect of the mesopores and mixed‐mode hydrophobic/ion‐exchange interactions, the OMSCFs were applied for rapid enrichment of endogenous peptides by using a miniaturized solid‐phase extraction format. The adsorption mechanism was studied, and the eluting solution was optimized with standard peptide/protein solutions and protein digests. Employing a successive three‐step elution strategy, followed by LC‐MS/MS analysis, led to excellent performance with this approach in the extraction and prefractionation of peptides from human serum.</p> </abstract>