A Photoreactive G‐Quadruplex Ligand Triggered by Green Light. Issue 6 (15th December 2014)

• Record Type: Journal Article
• Authors: Nadai, Matteo; Doria, Filippo; Germani, Luca; Richter, Sara N.; Freccero, Mauro
• Is Part Of: Chemistry. Volume 21:Issue 6(2015)
• Page Start: 2330
• Page End: 2334
• Publication Date: 2014-12-15
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201405215
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A photoreactive molecular dye targeting the G‐quadruplex nucleic acid (G4) of the human telomeric sequence Tel22, and several mutated analogues, was activated by green light (<italic>λ</italic>=532 nm). Highly selective covalent modification of G4 versus single‐stranded and double‐stranded DNA was achieved with efficiency up to 64 %. The phenoxyl radical was generated and detected by laser‐flash photolysis as a reactive intermediate that targeted loop thymine residues. These insights may suggest a non‐invasive tool for selective nucleic acid tagging and "pull‐down" cellular applications.</p> </abstract>