Biodegradation of chlorpyrifos and 3, 5, 6‐trichloro‐2‐pyridinol by a novel rhizobial strain Mesorhizobium sp. HN3. (7th March 2014)

• Record Type: Journal Article
• Authors: Jabeen, Hina; Iqbal, Samina; Anwar, Samina
• Is Part Of: Water and environment journal. Volume 29:Number 1(2015)
• Page Start: 151
• Page End: 160
• Publication Date: 2014-03-07
• Subjects: Sewage -- Periodicals; Water-supply -- Periodicals; Environmental management -- Periodicals; Water-supply engineering -- Periodicals; Pollution -- Periodicals; 628.1
• DOI: 10.1111/wej.12081
• Languages: English
• ISSNs: 1747-6585

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>A chlorpyrifos (CP) and 3, 5, 6‐trichloro‐2‐pyridinol (TCP) degrading bacterial strain, <italic>M</italic><italic>esorhizobium</italic> sp. HN3, was isolated and characterized. <italic>M</italic><italic>esorhizobium</italic> sp. HN3 degraded CP efficiently up to 400 mg/L initial concentration at wide range of temperatures (30–40°C) and pH (6.0–8.0). However, optimal degradation of CP was achieved at 37°C and neutral pH (7.0) at an initial inoculum density 2 × 10⁷ colony forming unit/mL of culture medium. Kinetic parameters for CP degradation by <italic>M</italic><italic>esorhizobium</italic> sp. HN3 were estimated at different initial concentrations. Cultures exhibited significant variation (<italic>P</italic> ≤ 0.05) in the specific growth rate (μ), cell mass formation rate (Q_X) and the substrate uptake rate (Q_S) during degradation of CP. The values of kinetic parameters increased up to 100 mg/L CP and decreased at higher concentration. Investigation of degradation metabolites indicated that CP is converted to diethylthiophosphate and TCP that leads to the formation of 3, 5, 6‐trichloro‐2‐methoxypyridine.</p> </abstract>