A Critical Role for Sox9 in Notch‐Induced Astrogliogenesis and Stem Cell Maintenance123. (20th March 2013)

• Record Type: Journal Article
• Authors: Martini, Simone; Bernoth, Kristina; Main, Heather; Ortega, German Dario Camargo; Lendahl, Urban; Just, Ursula; Schwanbeck, Ralf
• Is Part Of: Stem cells. Volume 31:Number 4(2013:Apr.)
• Page Start: 741
• Page End: 751
• Publication Date: 2013-03-20
• Subjects: Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.1320
• Languages: English
• ISSNs: 1066-5099

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Notch signaling is a key regulator of cell‐fate decisions and is essential for proper neuroectodermal development. There, it favors the formation of ectoderm, promotes maintenance of neural stem cells, inhibits differentiation into neurons, and commits neural progenitors to a glial fate. In this report, we explore downstream effects of Notch important for astroglial differentiation. Transient activation of Notch1 during early stages of neuroectodermal differentiation of embryonic stem cells resulted in an increase of neural stem cells, a reduction in neurons, an induction of astroglial cell differentiation, and an induction of neural crest (NC) development. Transient or continuous activation of Notch1 during neuroectodermal differentiation led to upregulation of <italic>Sox9</italic> expression. Knockdown of the Notch1‐induced <italic>Sox9</italic> expression reversed Notch1‐induced astroglial cell differentiation, increase in neural stem cells, and the decrease in neurons, whereas the Notch1 effects on NC development were hardly affected by knockdown of <italic>Sox9</italic> expression. These findings reveal a critical role for Notch‐mediated upregulation of <italic>Sox9</italic> in a select set of neural lineage determination steps controlled by Notch. S<sc>TEM</sc> C<sc>ELLS</sc><italic>2013;31:741–751</italic></p> </abstract>