Qualitative and Quantitative Analysis of Potentilla fulgens Roots by NMR, Matrix‐assisted Laser Desorption/Ionisation with Time‐of‐Flight MS, Electrospray Ionisation MS/MS and HPLC/UV. Issue 2 (16th December 2014)

• Record Type: Journal Article
• Authors: Choudhary, Alka; Radhika, Manukonda; Chatterjee, Anupam; Banerjee, Uttam Chand; Singh, Inder Pal
• Is Part Of: Phytochemical analysis. Volume 26:Issue 2(2015:Mar.)
• Page Start: 161
• Page End: 170
• Publication Date: 2014-12-16
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2547
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="pca2547-sec-0001" sec-type="section"> <title>Introduction</title> <p> <italic>Potentilla fulgens</italic> is a commonly used folk medicine by natives of northeast India, Nepal and Bhutan and is rich in polyphenolic and triterpene constituents.</p> </sec> <sec id="pca2547-sec-0002" sec-type="section"> <title>Objective</title> <p>To identify chemomarkers in the roots of <italic>P. fulgens</italic> by an interplay of <sup>13</sup>C‐NMR, matrix‐assisted laser desorption/ionisation with time‐of‐flight (MALDI/TOF) MS, electrospray ionisation (ESI) MS/MS and HPLC/UV.</p> </sec> <sec id="pca2547-sec-0003" sec-type="section"> <title>Material and methods</title> <p>The <sup>13</sup>C‐NMR spectrum of crude methanolic extract was recorded in deuterated dimethyl sulphoxide. For MALDI/TOF/MS analysis, 2, 5‐dihydroxybenzoic acid was used as the matrix. For determination of chemical constituents, two independent simple isocratic HPLC/UV methods for monomeric/oligomeric flavanols and triterpene acids were developed and validated.</p> </sec> <sec id="pca2547-sec-0004" sec-type="section"> <title>Results</title> <p>The <sup>13</sup>C‐NMR spectrum of the methanolic extract indicated the presence of B‐type oligomeric polyphenolics containing mainly epicatechin/catechin (epicat/cat) and epiafzelechin/afzelechin (epiafz/afz) as the monomeric units. Several isobaric monomeric and oligomeric flavanols and triterpenoids were tentatively identified by MALDI/TOF/MS and ESI/MS/MS. Fourteen compounds (four monomeric and five dimeric flavanols and five triterpene acids) were isolated using repeated column chromatography and semi‐preparative HPLC, and were quantitated using HPLC/UV.</p> </sec> <sec id="pca2547-sec-0005" sec-type="section"> <title>Conclusion</title> <p>It is evident from these analyses that roots of <italic>P. fulgens</italic> contain flavans, including oligomeric flavanols, as major constituents followed by triterpene acids. The methods described can be applied to other <italic>Potentilla</italic> species to identify their constituents. Copyright © 2014 John Wiley & Sons, Ltd.</p> </sec> </abstract>