Furfural and hydroxymethylfurfural tolerance in Escherichia coli ΔacrR regulatory mutants. (2nd July 2014)

• Record Type: Journal Article
• Authors: Luhe, Annette Lin; Lim, Chan Yuen; Gerken, Henri; Wu, Jinchuan; Zhao, Hua
• Is Part Of: Biotechnology and applied biochemistry. Volume 62:Number 1(2015:Jan./Feb.)
• Page Start: 32
• Page End: 36
• Publication Date: 2014-07-02
• Subjects: Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1232
• Languages: English
• ISSNs: 0885-4513

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The presence of the highly toxic furfural and hydroxymethylfurfural (HMF) in the hydrolysate of lignocellulosic biomass prompted the investigation of the <italic>Escherichia coli</italic> Δ<italic>acrR</italic> regulatory mutant for higher tolerance to these compounds, to facilitate the production of biofuels and biochemicals, and further biocatalytic conversions. In comparison with the parental strain, the regulatory mutant with the upregulated efflux pump AcrAB‐TolC produced moderately better growth and higher tolerance to concentrations of furfural and HMF between 1 and 2 g L⁻¹.</p> </abstract>