In Situ Analysis for Herbal Pieces of Aconitum Plants by Using Direct Analysis in Real Time Mass Spectrometry. Issue 2 (14th January 2015)

• Record Type: Journal Article
• Authors: Zhou, Feng; Zhu, Hongbin; Liu, Shu; Ma, Kang; Song, Fengrui; Liu, Zhiqiang
• Is Part Of: Chinese journal of chemistry. Volume 33:Issue 2(2015:Feb.)
• Page Start: 241
• Page End: 246
• Publication Date: 2015-01-14
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cjoc.201400692
• Languages: English
• ISSNs: 1001-604X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>In this study, an extend application was developed to <italic>in situ</italic> analyze the herbal pieces of <italic>Aconitum</italic> plants by Direct Analysis in Real Time Mass Spectrometry (DART‐MS). Nearly all aconitine‐type alkaloids can be desorbed and ionized in this method, including diester diterpenoid aconitines (DDAs), monoester diterpenoid aconitines (MDAs) and some other diterpenoid aconitines. The spectra of <italic>in situ </italic>analysis for the herbal pieces of aconitum plants are similar with that of their extracts. <italic>Radix Aconiti</italic> and <italic>Radix Aconiti Kusnezoffii</italic> can be distinguished from each other by the intensity differences of character fragment ions from MDAs, such as <italic>m</italic>/<italic>z</italic> 586, 572, and 556. The qualified and unqualified herbal pieces can be also identified by the relative abundances of DDAs. The RSD of the relative abundances of some character ions at <italic>m</italic>/<italic>z</italic> 556, 586, and 590 were 13.53%, 4.03%, and 12.03%, respectively. So this <italic>in situ </italic>analytical method can identify both the types of Aconitum preparata and their quality. It provides the following advantages in the analysis of Chinese herbs: fast detection without much pretreatment, high‐throughput analysis, and reduction of pollution without any organic solvent.</p> </abstract>