8-pCPT, an Epac activator, impairs conditioned place preference based on nucleus accumbens amphetamine in rats. Issue 2 (1st August 2013)

• Record Type: Journal Article
• Authors: Park, Sung Woo; Roohbakhsh, Ali; Beninger, Richard J.
• Is Part Of: Acta neuropsychiatrica. Volume 26:Issue 2(2014:Apr.)
• Page Start: 104
• Page End: 111
• Publication Date: 2013-08-01
• Subjects: Neuropsychiatry -- Periodicals; 616.89
• DOI: 10.1017/neu.2013.37
• Languages: English
• ISSNs: 0924-2708

Abstract

<abstract abstract-type="normal"> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <sec id="abs1" sec-type="general"> <title>Objectives</title> <p>Dopamine receptor-mediated 3′, 5′-cyclic adenosine monophosphate (cAMP)-dependent intracellular signalling is important for reward-related learning. cAMP activates cAMP-dependent protein kinase (PKA) and exchange protein directly activated by cAMP (Epac). We tested the hypothesis that reward-related learning may be mediated by Epac.</p> </sec> <sec id="abs2" sec-type="methods"> <title>Methods</title> <p>We evaluated conditioned place preference (CPP) on the basis of nucleus accumbens (NAc) injections of amphetamine (20 μg/0.5 μl/side) plus Sp-adenosine 3′, 5′-cyclic monophosphorothioate triethylamanine (Sp-cAMPS) (0.1, 1.0, 10, 15, 20 μg/0.5 μl/side), an activator of both PKA and Epac, or amphetamine (20 μg) plus 8-(4-chlorophenylthio)-2′-<italic>O</italic>-methyladenosine-3′, 5′-cyclic monophosphate (8-pCPT) (0.73, 1.27, 1.45, 2.89, 5.78, 11.56 μg/0.5 μl/side), an activator of Epac.</p> </sec> <sec id="abs3" sec-type="results"> <title>Results</title> <p>In agreement with previous results, Sp-cAMPS dose-dependently impaired CPP. 8-pCPT impaired CPP at one dose (1.45 μg/0.5 μl/side) and we replicated this effect three times.</p> </sec> <sec id="abs4" sec-type="conclusion"> <title>Conclusion</title> <p>The results implicate Epac in the acquisition of reward-related learning.</p> </sec> </abstract>