Activation of caspase-9 and its influencing factors in beef during conditioning. (5th December 2013)

• Record Type: Journal Article
• Authors: Cao, J.; Zhou, G.; Liu, Y.; Liao, G.; Zhang, Q.; Ye, K.; Pan, D.; Ou, C.
• Is Part Of: Animal. Volume 8:Number 3(2014:Mar.)
• Page Start: 504
• Page End: 509
• Publication Date: 2013-12-05
• Subjects: Animal breeding -- Periodicals; Animal genetics -- Periodicals; Animal nutrition -- Periodicals; Animal physiology -- Periodicals; Environmental sciences -- Periodicals; 636.005
• DOI: 10.1017/S175173111300219X
• Languages: English
• ISSNs: 1751-7311

Abstract

<abstract abstract-type="normal"> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <p>To study the activation of caspase-9 and its potential influence in conditioning, longissimus thoracis (LT), semitendinosus (STN) and psoas minor (PMi) muscles were used to analyze the ratio of pro-apoptotic bax to anti-apoptotic bcl-2 in fresh tissues and observe the changes in ATP, cytosolic cytochrome <italic>c</italic> and caspase-9 activity levels during storage at 4°C. Caspase-9 activity at 5 h is higher than the activity at 0 and 24 h in the muscles (<italic>P</italic><0.001). The ATP content decreased between 0 and 3 h, between 8 and 14 h in the PMi and LT muscles (<italic>P</italic><0.0001), whereas between 0 and 5 h, between 8 and 14 h in the STN muscle (<italic>P</italic><0.0001). There is 60.2%, 55.3% and 43.1% available ATP in the STN, LT and PMi muscles at 5 h, respectively. The cytosolic cytochrome <italic>c</italic> level increased during 5 and 24 h storage in the LT and PMi muscles (<italic>P</italic><0.0001), during 5 and 96 h in the STN muscle (<italic>P</italic><0.0001). The cytosolic cytochrome <italic>c</italic> at 24 h (<italic>P</italic><0.001) and ratio of bax to bcl-2 (<italic>P</italic><0.05) was higher in the PMi than in other muscles. We concluded that the increase in cytosolic cytochrome <italic>c</italic> and available intracellular ATP should be responsible for the increase in caspase-9 activity; the activation of caspase-9 could be limited by the subsequent depletion of ATP; the <italic>postmortem</italic> release level of cytochrome <italic>c</italic> could be determined by the ratio of bax to bcl-2 in fresh tissues.</p> </abstract>