Complex assembly, crystallization and preliminary X‐ray crystallographic analysis of the bovine CD8αα–BoLA‐2*02201 complex. Issue 6 (1st June 2014)

• Record Type: Journal Article
• Authors: Wang, Zhenbao; Chen, Rong; Tariq, Mansoor; Jiang, Bo; Chen, Zhaosan; Xia, Chun
• Is Part Of: Acta crystallographica. Volume 70:Issue 6(2014:Jun.)
• Page Start: 742
• Page End: 746
• Publication Date: 2014-06-01
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X14008838
• Languages: English
• ISSNs: 2053-230X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>In order to clarify the structural characteristics of the bovine MHC class I molecule (BoLA‐I) complexed with CD8αα (CD8αα–BoLA‐I), bovine CD8αα, BoLA‐I (BoLA‐2*02201) and β₂m were expressed and purified, and were then assembled with a peptide derived from <italic>Foot‐and‐mouth disease virus</italic> (FMDV‐VP1_YY9) and crystallized. The crystal diffracted to 1.7 Å resolution and belonged to space group <italic>P</italic>2₁, with unit‐cell parameters <italic>a</italic> = 53.9, <italic>b</italic> = 103.8, <italic>c</italic> = 61.8 Å, α = γ = 90, β = 96°. The asymmetric unit contained one complex, with a Matthews coefficient of 2.41 ų Da⁻¹ and a solvent content of 48.9%. The rotation‐function <italic>Z</italic>‐score and translation‐function <italic>Z</italic>‐score for molecular replacement were 3.4 and 8.9, respectively. In addition, SDS–PAGE analysis of CD8αα–BoLA‐I crystals showed three bands corresponding to the molecular weights of BoLA‐I heavy chain, β₂m and CD8α. The structure of the CD8αα–BoLA‐I complex should be helpful in obtaining insight into the interaction between bovine CD8αα and MHC class I molecules. Structure determination of BoLA‐2*02201–FMDV‐VP1_YY9 will be useful in the design of vaccines for foot‐and‐mouth disease.</p> </abstract>