Characterization of HMW glutenin subunit Bx7OE and its distribution in common wheat and related species. Issue 2 (August 2014)

• Record Type: Journal Article
• Authors: Li, Jie; Han, Caixia; Zhen, Shoumin; Li, Xiaohui; Yan, Yueming
• Is Part Of: Plant genetic resources. Volume 12:Issue 2(2014)
• Page Start: 191
• Page End: 198
• Publication Date: 2014-08
• Subjects: Plant genetics -- Periodicals; Plant breeding -- Periodicals; 632.523
• DOI: 10.1017/S1479262113000476
• Languages: English
• ISSNs: 1479-2621

Abstract

<abstract abstract-type="normal"> <title> <x content-type="archive" xml:space="preserve">Abstract</x> </title> <p>The overexpression of wheat Bx7 subunit (Bx7<sup>OE</sup>) encoded by the <italic>Glu-B1al</italic> allele is originated from a duplication event of the <italic>Bx7</italic> gene, and has a positive effect on gluten strength. Thus, it is an important genetic resource for wheat quality improvement. In this study, the Bx7<sup>OE</sup> subunit from a large number of bread wheat and related species was characterized by sodium dodecyl sulphate–polyacrylamide gel electrophoresis, reversed-phase high-performance liquid chromatography (RP-HPLC) and Sequence-Tagged sites (STS) markers. Only 31 bread wheat varieties were found to carry Bx7<sup>OE</sup>. RP-HPLC quantification analysis revealed that the mean proportion of the Bx7<sup>OE</sup> subunit to the total amount of high-molecular-weight glutenin subunits among the 31 bread wheat varieties was 41.8%, which is much higher than that of varieties with the normal Bx7 subunit (generally at 30%). Flour quality analysis of seven representative varieties with Bx7<sup>OE</sup> and three with the normal Bx7 subunit showed that the varieties with Bx7<sup>OE</sup> generally displayed better gluten strength than those with the normal Bx7 subunit. STS markers demonstrated that, in addition to the 31 bread wheat varieties with Bx7<sup>OE</sup>, no PCR products were obtained from the related <italic>Triticum</italic> and <italic>Aegilops</italic> species. This suggests that the retroelement-mediated recombination event at the <italic>Glu-B1</italic> locus could have occurred more recently, later than the formation of hexaploid wheat. The Bx7<sup>OE</sup> subunit is mainly distributed in some bread wheat varieties from American countries with a low frequency, which is of particular importance for the quality improvement of wheat gluten.</p> </abstract>