Interactions of polydispersed single-walled carbon nanotubes with T cells resulting in downregulation of allogeneic CTL responses in vitro and in vivo. Issue 8 (December 2012)

• Record Type: Journal Article
• Authors: Alam, Anwar; Sachar, Sumedha; Puri, Niti; Saxena, Rajiv K.
• Is Part Of: Nanotoxicology. Volume 7:Issue 8(2013:Dec.)
• Page Start: 1351
• Page End: 1360
• Publication Date: 2012-12
• Subjects: Toxicology -- Periodicals; 615.9
• DOI: 10.3109/17435390.2012.739666
• Languages: English
• ISSNs: 1743-5390

Abstract

<abstract> <title>Abstract</title> <p>Addition of polydispersed acid functionalised single-walled carbon nanotubes (AF-SWCNTs) significantly suppressed alloimmune cytotoxic T cell (CTL) response generated in a mixed lymphocyte reaction (MLR) between spleen cells from C57BL/6 (H-2^b) and BALB/c (H-2^d) mice. AF-SWCNTs treatment also decreased CD69 expression, enhanced apoptotic response in T cells and reduced significantly the recovery of live CD4⁺ and CD8⁺ T cells from MLR cultures. A two to threefold increase was noticed in the binding/uptake of AF-SWCNTs by T cells in MLR cultures as compared with control cultured T cells. Confocal microscopy confirmed the internalization of AF-SWCNTs by live CD8⁺ T cells in MLR cultures. Administration of AF-SWCNTs suppressed the generation of anti-P815 CTL response <italic>in</italic> C57BL/6 mice and the recovery of T-cell populations from the spleens. The results demonstrate a suppressive effect of AF-SWCNTs on CTL response and provide an insight into the mechanism of this suppression.</p> </abstract>