The effect of M‐phase stage‐dependent kinase inhibitors on inositol 1, 4, 5‐trisphosphate receptor 1 (IP3R1) expression and localization in pig oocytes. (3rd September 2014)

• Record Type: Journal Article
• Authors: Sathanawongs, Anucha; Fujiwara, Katsuyoshi; Kato, Tsubasa; Hirose, Masahiko; Kamoshita, Maki; Wojcikiewicz, Richard J. H.; Parys, Jan B.; Ito, Junya; Kashiwazaki, Naomi
• Is Part Of: Animal science journal. Volume 86:Number 2(2015:Feb.)
• Page Start: 138
• Page End: 147
• Publication Date: 2014-09-03
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12258
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>At fertilization, inositol 1, 4, 5‐trisphosphate receptor type 1 (IP<sub>3</sub>R1) has a crucial role in Ca<sup>2+</sup> release in mammals. Expression levels, localization and phosphorylation of IP<sub>3</sub>R1 are important for its function, but it still remains unclear which molecule(s) regulates IP<sub>3</sub>R1 behavior in pig oocytes. We examined whether there was a difference in localization of IP<sub>3</sub>R1 after <italic>in vitro</italic> or <italic>in vivo</italic> maturation of pig oocytes. In mouse oocytes, large clusters of IP<sub>3</sub>R1 were formed in the cortex of the oocyte except in a ring‐shaped band of cortex adjacent to the spindle. However, no such clusters of IP<sub>3</sub>R1 were observed in pig oocytes and there was no difference in its localization between <italic>in vitro</italic> and <italic>in vivo</italic> matured oocytes. We next tried to clarify which factor(s) regulates IP<sub>3</sub>R1 localization, phosphorylation and expression using M‐phase stage‐dependent kinase inhibitors. Our results show that treatments with roscovitine (p34<sup>cdc2</sup> kinase inhibitor) or U0126 (mitogen‐activated protein kinase inhibitor) did not affect IP<sub>3</sub>R1 expression or localization in pig oocytes, although the latter strongly inhibited phosphorylation. However, treatment with BI‐2536, an inhibitor of polo‐like kinase 1 (Plk1), dramatically decreased the expression level of IP<sub>3</sub>R1 in pig oocytes in a dose‐dependent manner. From these results, it is suggested that Plk1 is involved in the regulation of IP<sub>3</sub>R1 expression in pig oocytes.</p> </abstract>