Candida albicans aspects of novel silane system–coated titanium and zirconia implant surfaces. Issue 3 (23rd January 2014)

• Record Type: Journal Article
• Authors: Villard, Nicolas; Seneviratne, Chaminda; Tsoi, James Kit Hon; Heinonen, Markku; Matinlinna, Jukka
• Is Part Of: Clinical oral implants research. Volume 26:Issue 3(2015:Mar.)
• Page Start: 332
• Page End: 341
• Publication Date: 2014-01-23
• Subjects: Dental implants -- Research -- Periodicals; 617.69
• DOI: 10.1111/clr.12338
• Languages: English
• ISSNs: 0905-7161

Abstract

<abstract abstract-type="main" id="clr12338-abs-0001"> <title>Abstract</title> <sec id="clr12338-sec-0001" sec-type="section"> <title>Objectives</title> <p>The aim of the present study was to evaluate the effect of novel silane system coatings on zirconia and titanium implant surfaces and the attachment of the fungal pathogen <italic>Candida albicans</italic>.</p> </sec> <sec id="clr12338-sec-0002" sec-type="section"> <title>Materials and methods</title> <p>Titanium and zirconia specimens were silica‐coated and silanized either with a commercial silane primer (RelyX Ceramic Primer™, 3M ESPE) or a novel silane system primer. The novel silane system primer was a blend of 1.0 vol% 3‐acryloxypropyltrimethoxysilane and 0.3 vol% bis‐1, 2‐(triethoxysilyl)ethane diluted in acidified ethanol‐water solvent. The surface roughness (R<sub>a</sub>), the surface free energy and the chemical composition of substrate surfaces after treatments were evaluated. <italic>C. albcans</italic> biofilms were developed on silica‐coated + silanized surfaces during 48 h of incubation time. Colony forming units (CFU) and real‐time PCR (RT‐PCR) quantified the cells on the material surfaces. Statistical analyses were carried out by 1‐way ANOVA, Tukey <italic>post hoc</italic> and Games Howell <italic>post hoc</italic> test at 5% significance level <italic>(p)</italic>.</p> </sec> <sec id="clr12338-sec-0003" sec-type="section"> <title>Results</title> <p>On zirconia and titanium surfaces, the R<sub>a</sub> and the chemical composition of the specimens were equal (<italic>P</italic> < 0.05). The surface free energy was decreased on titanium specimens and increased on zirconia specimen after silanization. CFU of <italic>C. albicans</italic> was significantly lower on zirconia coated with RelyX Ceramic Primer™, (<italic>P </italic><<italic> </italic>0.001) and on titanium coated with both silanes (<italic>P </italic>=<italic> </italic>0.002). RT‐PCR revealed no differences between the mean quantities of <italic>C. albicans</italic> (<italic>P </italic>≥<italic> </italic>0.067).</p> </sec> <sec id="clr12338-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Silica‐coating and silanization had modified the titanium and zirconia surfaces significantly. Both the control and experimental silane primers might inhibit the biofilm formation of <italic>C. albicans</italic>.</p> </sec> </abstract>