Flow‐injection chemiluminescence method to detect a β2 adrenergic agonist. (16th May 2014)

• Record Type: Journal Article
• Authors: Zhang, Guangbin; Tang, Yuhai; Shang, Jian; Wang, Zhongcheng; Yu, Hua; Du, Wei; Fu, Qiang
• Is Part Of: Luminescence. Volume 30:Number 1(2015:Jan./Feb.)
• Page Start: 102
• Page End: 109
• Publication Date: 2014-05-16
• Subjects: Luminescence -- Periodicals; Bioluminescence -- Periodicals; Chemiluminescence -- Periodicals; Luminescence -- Periodicals; 535.35
• DOI: 10.1002/bio.2698
• Languages: English
• ISSNs: 1522-7235

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>A new method for the detection of β<sub>2</sub> adrenergic agonists was developed based on the chemiluminescence (CL) reaction of β<sub>2</sub> adrenergic agonist with potassium ferricyanide–luminol CL. The effect of β<sub>2</sub> adrenergic agonists including isoprenaline hydrochloride, salbutamol sulfate, terbutaline sulfate and ractopamine on the CL intensity of potassium ferricyanide–luminol was discovered. Detection of the β<sub>2</sub> adrenergic agonist was carried out in a flow system. Using uniform design experimentation, the influence factors of CL were optimized. The optimal experimental conditions were 1 mmol/L of potassium ferricyanide, 10 µmol/L of luminol, 1.2 mmol/L of sodium hydroxide, a flow speed of 2.6 mL/min and a distance of 1.2 cm from 'Y<sub>2</sub>' to the flow cell. The linear ranges and limit of detection were 10–100 and 5 ng/mL for isoprenaline hydrochloride, 20–100 and 5 ng/mL for salbutamol sulfate, 8–200 and 1 ng/mL for terbutaline sulfate, 20–100 and 4 ng/mL for ractopamine, respectively. The proposed method allowed 200 injections/h with excellent repeatability and precision. It was successfully applied to the determination of three β<sub>2</sub> adrenergic agonists in commercial pharmaceutical formulations with recoveries in the range of 96.8–98.5%. The possible CL reaction mechanism of potassium ferricyanide–luminol–β<sub>2</sub> adrenergic agonist was discussed from the UV/vis spectra. Copyright © 2014 John Wiley & Sons, Ltd.</p> </abstract>