Discrimination of p53 immunohistochemistry‐positive tumors by its staining pattern in gastric cancer. (30th October 2014)

• Record Type: Journal Article
• Authors: Ando, Koji; Oki, Eiji; Saeki, Hiroshi; Yan, Zhao; Tsuda, Yasuo; Hidaka, Gen; Kasagi, Yuta; Otsu, Hajime; Kawano, Hiroyuki; Kitao, Hiroyuki; Morita, Masaru; Maehara, Yoshihiko
• Is Part Of: Cancer medicine. Volume 4:Number 1(2015:Jan.)
• Page Start: 75
• Page End: 83
• Publication Date: 2014-10-30
• Subjects: 616.994005
• DOI: 10.1002/cam4.346
• Languages: English
• ISSNs: 2045-7634

Abstract

<abstract abstract-type="main" id="cam4346-abs-0001"> <title>Abstract</title> <p>Immunohistochemistry staining of p53 is a cheap and simple method to detect aberrant function of p53. However, there are some discrepancies between the result of immunohistochemistry staining and mutation analysis. This study attempted to find a new definition of p53 staining by its staining pattern. Immunohistochemistry staining of p53 and <italic>TP53</italic> gene mutation analysis were performed in 148 gastric cancer patients. Also SNP‐CGH array analysis was conducted to four cases. Positive staining of p53 was observed in 88 (59.5%) tumors. Tumors with positive p53 staining showed malignant features compared to negative tumors. Mutation of <italic>TP53</italic> gene was observed in 29 (19.6%) tumors with higher age and differentiated type. In positive p53 tumors, two types could be distinguished; aberrant type and scattered type. With comparison to <italic>TP53</italic> gene mutation analysis, all the scattered type had wild‐type <italic>TP53</italic> gene (<italic>P</italic> = 0.0003). SNP‐CGH array showed that scattered‐type tumors had no change in the structure of chromosome 17. P53‐scattered‐type staining tumors may reflect a functionally active nonmutated <italic>TP53</italic> gene. In interpretation of p53 immunohistochemistry staining, distinguishing p53‐positive tumors by their staining pattern may be important in gastric cancer.</p> </abstract>