High‐Resolution N6‐Methyladenosine (m6A) Map Using Photo‐Crosslinking‐Assisted m6A Sequencing1. (9th December 2014)

• Record Type: Journal Article
• Authors: Chen, Kai; Lu, Zhike; Wang, Xiao; Fu, Ye; Luo, Guan‐Zheng; Liu, Nian; Han, Dali; Dominissini, Dan; Dai, Qing; Pan, Tao; He, Chuan
• Is Part Of: Angewandte Chemie. Volume 127:Number 5(2015)
• Page Start: 1607
• Page End: 1610
• Publication Date: 2014-12-09
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201410647
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>N⁶‐methyladenosine (m⁶A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m⁶A modification remain limited. We present here a photo‐crosslinking‐assisted m⁶A sequencing strategy (PA‐m⁶A‐seq) to more accurately define sites with m⁶A modification. Using this strategy, we obtained a high‐resolution map of m⁶A in a human transcriptome. The map resembles the general distribution pattern observed previously, and reveals new m⁶A sites at base resolution. Our results provide insight into the relationship between the methylation regions and the binding sites of RNA‐binding proteins.</p> </abstract>