Biochemical and histopathological evidence on the beneficial effects of Tragopogon graminifolius in TNBS-induced colitis. (March 2015)

• Record Type: Journal Article
• Authors: Farzaei, Mohammad Hosein; Ghasemi-Niri, Seyedeh Farnaz; Abdolghafari, Amir Hosein; Baeeri, Maryam; Khanavi, Mahnaz; Navaei-Nigjeh, Mona; Abdollahi, Mohammad; Rahimi, Roja
• Is Part Of: Pharmaceutical biology. Volume 53:Number 3(2015:Mar.)
• Page Start: 429
• Page End: 436
• Publication Date: 2015-03
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.923004
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context: Tragopogon graminifolius</italic> DC. (Compositae) (TG) has been proposed as an efficacious remedy for gastrointestinal ulcers in Iranian traditional medicine.</p> <p> <italic>Objective</italic>: The present study evaluates the efficacy of TG on experimental colitis and the responsible mechanisms.</p> <p> <italic>Materials and methods</italic>: After induction of IBD by 2, 4, 6-trinitrobenzenesulfonic acid (TNBS), rats received standardized ethanol extract of TG aerial part at 20, 30, or 50 mg/kg/d orally. After 12 d, the rats were sacrificed and the colon was removed and assessed for macroscopic and microscopic changes. Also, tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), total antioxidant capacity, myeloperoxidase (MPO), and lipid peroxidation (LPO) were measured in the colon homogenate.</p> <p> <italic>Result</italic>: TG extract significantly reduced macroscopic and microscopic scores of colitis with ED<sub>50</sub> values of 23 and 39 mg/kg, respectively. MPO was significantly reduced in all plant extract groups with an ED<sub>50</sub> value of 41 mg/kg. The ED<sub>50</sub> values of extract for inhibition of TNF-α and LPO were 44 and 93 mg/kg, respectively. IL-1β significantly decreased by 50 mg/kg of TG extract (ED<sub>50</sub> = 57 mg/kg). Total antioxidant power markedly increased by 50 mg/kg group (ED<sub>50</sub> = 43 mg/kg).</p> <p> <italic>Discussion</italic>: TG exhibited efficacy on TNBS-induced colitis via anti-inflammatory, immunomodulatory, antioxidant, and mucosal healing properties.</p> <p> <italic>Conclusion</italic>: TG possesses promising healing function on colitis. Clinical trials are warranted to prove its efficacy and tolerability in IBD.</p> </abstract>