Bioassay guided fractionation and identification of active anti-inflammatory constituent from Delonix elata flowers using RAW 264.7 cells. (February 2015)

• Record Type: Journal Article
• Authors: Saravanan, S.; Hairul Islam, V. I.; David, H. A.; Lakshmi Sundaram, R.; Chellappandian, M.; Balakrishna, K.; Rajendran, R.; Vijayaraghavan, P.; Gabriel Paulraj, M.; Ignacimuthu, S.
• Is Part Of: Pharmaceutical biology. Volume 53:Number 2(2015:Feb.)
• Page Start: 174
• Page End: 184
• Publication Date: 2015-02
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.913067
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: <italic>Delonix elata</italic> (L.) Gamble (Fabaceae) has been used in the Indian traditional medicine system to treat rheumatism and inflammation.</p> <p> <italic>Aim</italic>: To assess the anti-inflammatory effect of <italic>Delonix elata</italic> flowers and to isolate the active principle.</p> <p> <italic>Materials and methods</italic>: The prompt anti-inflammatory constituent was isolated from <italic>Delonix elata</italic> flower extracts using bioassay guided fractionation in liposaccharide (LPS) stimulated RAW 264.7 macrophage cell line. The anti-inflammatory activity of extracts/fractions/sub-fractions/compounds (10, 25, and 50 µg/ml) was evaluated by estimating the levels of nitric oxide (NO), TNF-α, and IL-1β after 24 h of LPS induction (1 μg/ml). The isolated active compound was subjected to NMR, IR, and UV analyses for structure determination.</p> <p> <italic>Results</italic>: In an attempt to search for anti-inflammatory constituents, the active pure principle was isolated and crystallized as a white compound from <italic>Delonix elata</italic> flowers methanol extract. This active compound (50 µg/ml) decreased the release of inflammatory mediators levels such as NO (0.263 ± 0.03 µM), TNFα (160.20 ± 17.57 pg/ml), and IL-1β (285.79 ± 15.16 pg/ml) significantly (<italic>p</italic> < 0.05); when compared to the levels of NO (0.774 ± 0.08 µM), TNFα (501.71 ± 25.14 pg/ml), and IL-1β (712.68 ± 52.25 pg/ml) from LPS-stimulated macrophage cells. The active compound was confirmed as hesperidin with NMR, IR, and UV spectroscopy data. This is the first report of this compound from <italic>Delonix elata</italic> flowers.</p> <p> <italic>Conclusion</italic>: The findings of the study support the traditional use of <italic>Delonix elata</italic> flowers to treat inflammation.</p> </abstract>