Inhibitory function of P-selectin-mediated leukocyte adhesion by the polysaccharides from Sanguisorba officinalis. (March 2015)

• Record Type: Journal Article
• Authors: Tong, Haibin; Song, Jianxi; Zhang, Zhuorui; Mao, Dirui; Sun, Guangren; Jiang, Guiquan
• Is Part Of: Pharmaceutical biology. Volume 53:Number 3(2015:Mar.)
• Page Start: 345
• Page End: 349
• Publication Date: 2015-03
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.919597
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: P-selectin is a promising target for inflammatory-related diseases. Polysaccharides are the active ingredients of <italic>Sanguisorba officinalis</italic> L. (Rosaceae) responsible for its anti-inflammatory activities; however, the molecular mechanism is not clear yet.</p> <p> <italic>Objective</italic>: This study evaluates the effects of polysaccharides (SOPs) from <italic>Sanguisorba officinalis</italic> on their antagonistic function against P-selectin-mediated leukocyte adhesion.</p> <p> <italic>Materials and methods</italic>: The antagonistic function of SOPs was investigated by flow cytometry and static adhesion assay at the concentrations of 25 and 100 μg/ml. The dynamic interaction between HL-60 cells and CHO-P cell monolayer treated with SOPs (25 and 100 μg/ml) was analyzed in a parallel plate flow chamber, and quantitatively calculated by ImageJ software (NIH, Bethesda, MD). <italic>In vitro</italic> protein binding assay was carried out to evaluate the blocking effects of SOPs (25 and 100 μg/ml) on the interaction between P-selectin and PSGL-1.</p> <p> <italic>Results</italic>: SOPs-treatment (100 μg/ml) significantly reduced the percentage of HL-60 cells binding to P-selectin (<italic>p</italic> < 0.01) determined by flow cytometry. In addition, SOPs (25 and 100 μg/ml) markedly blocked the adhesion between HL-60 cells and CHO-P cells under static condition, and the inhibitory rates reached 39.9% and 71.2%, respectively. Compared with the positive control group, SOPs-treatment (25 and 100 μg/ml) significantly reduced the percentage of HL-60 cells rolling on CHO-P cell monolayers by 43.5% and 75.2%, respectively. Protein binding assay showed the interaction between P-selectin and PSGL-1 was significantly blocked by SOPs.</p> <p> <italic>Discussion and conclusion</italic>: SOPs possess a significant antagonistic function against P-selectin-mediated leukocyte adhesion, and SOPs could be considered as a promising candidate for amelioration of inflammation-related diseases.</p> </abstract>