Methy-sens Comet assay and DNMTs transcriptional analysis as a combined approach in epigenotoxicology. (February 2015)

• Record Type: Journal Article
• Authors: Perotti, Alessio; Rossi, Valeria; Mutti, Antonio; Buschini, Annamaria
• Is Part Of: Biomarkers. Volume 20:Number 1(2015)
• Page Start: 64
• Page End: 70
• Publication Date: 2015-02
• Subjects: Biochemical markers -- Periodicals; 610.28
• DOI: 10.3109/1354750X.2014.992813
• Languages: English
• ISSNs: 1354-750X

Abstract

<abstract> <title>Abstract</title> <p>Epigenotoxicology needs simple and fast tools to assess xenobiotic epigenetic load. This work proposes a comet assay modification designed to detect global methylation changes (Methy-sens Comet) through enzymatic digestion with two restriction enzymes (HpaII, MspI). In the methylation-sensitive protocol tested for repeatability on A549 cells, nickel chloride induced hypermethylation and decitabine-induced hypomethylation. A concomitant assessment of DNA methyltransferases (DNMTs) genes transcriptional levels has been performed, to implement a multifunctional approach to epigenotoxicology. Methy-sens Comet showed a general good repeatability and sensitivity to methylation changes while DNMTs transcriptional levels granted additional proof of xenobiotic-induced impairment of methylome maintenance.</p> </abstract>