DevR PCR for the Diagnosis of Intraocular Tuberculosis. (February 2015)

• Record Type: Journal Article
• Authors: Kataria, Pankaj; Kumar, Abiraj; Bansal, Reema; Sharma, Aman; Gupta, Vishali; Gupta, Amod; Singh, Ramandeep; Sharma, Kusum
• Is Part Of: Ocular immunology and inflammation. Volume 23:Number 1(2015)
• Page Start: 47
• Page End: 52
• Publication Date: 2015-02
• Subjects: Eye -- Diseases -- Immunological aspects -- Periodicals; Eye -- Inflammation -- Periodicals; 617.7
• DOI: 10.3109/09273948.2014.981550
• Languages: English
• ISSNs: 0927-3948

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To compare the efficacy of devR and MPB64 PCR in the diagnosis of intraocular tuberculosis.</p> <p> <italic>Methods</italic>: Prospective, nonrandomized study. Seventy-five patients were enrolled in 3 groups. Group A had 25 patients with presumed intraocular tubercular uveitis, group B had 25 controls with specific uveitis other than tubercular uveitis, and group C included 25 non-uveitic negative controls. The undiluted vitreous/aqueous samples were collected and subjected to PCR assay for devR and MPB64 gene sequence of <italic>Mycobacterium tuberculosis</italic> (MTB) to detect sensitivity and specificity.</p> <p> <italic>Results</italic>: devR PCR was positive in 16 (64%) out of 25 patients with presumed tubercular uveitis. MPB64 PCR was positive in 18 (72%) out of 25 patients with presumed tubercular uveitis. The sensitivity and specificity of devR were 64 and 100%, respectively. The sensitivity and specificity of MPB64 PCR were 72 and 100%, respectively.</p> <p> <italic>Conclusion</italic>: devR PCR is not a better tool than MPB64 PCR for diagnosing intraocular tuberculosis.</p> </abstract>