Peripheral Blood‐Derived Endothelial Progenitor Cells Enhance Vertical Bone Formation. (30th April 2013)

• Record Type: Journal Article
• Authors: Zigdon‐Giladi, Hadar; Bick, Tova; Morgan, Elise F.; Lewinson, Dina; Machtei, Eli E.
• Is Part Of: Clinical implant dentistry and related research. Volume 17:Number 1(2015:Feb.)
• Page Start: 83
• Page End: 92
• Publication Date: 2013-04-30
• Subjects: Dental implants -- Periodicals; Dental Implantation -- Periodicals; Dental Implants -- Periodicals; 617.693
• DOI: 10.1111/cid.12078
• Languages: English
• ISSNs: 1523-0899

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="cid12078-sec-0001" sec-type="section"> <title>Background</title> <p>This study presents a novel cell‐based approach for extra‐cortical bone regeneration.</p> </sec> <sec id="cid12078-sec-0002" sec-type="section"> <title>Objective</title> <p>To enhance vertical bone formation by combining guided bone regeneration and transplantation of peripheral blood‐derived endothelial progenitor cells (EPCs) in a rat calvaria model.</p> </sec> <sec id="cid12078-sec-0003" sec-type="section"> <title>Materials and Methods</title> <p>EPCs were isolated from peripheral blood of inbred rats. Gold domes (7 mm radius, 5 mm height) were filled with β‐tricalcium phosphate (βTCP) mixed with 5 × 10<sup>5</sup> EPC. Domes filled with βTCP served as control (CNT). Rats were sacrificed after 3 months. Vertical bone augmentation was analyzed using histology, histomorphometry, and microcomputed tomography (μCT).</p> </sec> <sec id="cid12078-sec-0004" sec-type="section"> <title>Results</title> <p>In all rats, hard tissue filled the space under the dome. Histomorphometric analysis revealed that EPC transplantations doubled vertical bone height (EPC 4.04 ± 0.22 mm vs CNT 2.29 ± 0.22 mm, <italic>p</italic> ≤ .001). EPC also caused ∼50% increase in bone area fraction (EPC 47.3 ± 3.1% vs CNT 31.1 ± 2.7%, <italic>p</italic> ≤ .003). μCT results also showed that bone volume fraction (BV/TV) was higher in EPC group (<italic>p</italic> = .0169). In both groups, BV/TV declined from the bottom to the top of the samples. No differences in tissue mineral density were found between EPC and CNT groups.</p> </sec> <sec id="cid12078-sec-0005" sec-type="section"> <title>Conclusion</title> <p>EPC transplantation significantly improved bone formation especially in the areas that are remote from the original bone.</p> </sec> </abstract>