Development of a sensitive ultra high performance liquid chromatography with tandem mass spectrometry method for the simultaneous quantification of nine active compounds in rat plasma and its application to a pharmacokinetic study after administration of Viscum coloratum extracts. Issue 3 (23rd December 2014)

• Record Type: Journal Article
• Authors: Fan, Rong‐Hua; Ding, Wei; Ma, Yu‐Ying; Lin, Hong‐Li; Men, Lei; Duan, Meng‐Meng; Zhao, Yun‐Li; Yu, Zhi‐Guo
• Is Part Of: Journal of separation science. Volume 38:Issue 3(2015:Feb.)
• Page Start: 530
• Page End: 540
• Publication Date: 2014-12-23
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201400804
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A simple, specific, and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous quantification of nine compounds including a new compound, rhamnazin‐3‐<italic>Ο</italic>‐β‐<sc>d</sc>‐(6″‐β‐hydroxy‐β‐methyglutaryl)<italic>‐</italic>β‐<sc>d</sc>‐glucoside‐4′<italic>‐Ο‐</italic>β‐<sc>d</sc>‐glucoside, in rat plasma using baicalin as an internal standard. The plasma samples were pretreated and extracted by protein precipitation with 0.2% formic acid in acetonitrile. The analytes were separated on a Thermo Syncronis C<sub>18</sub> column by gradient elution with a mobile phase consisting of acetonitrile and 0.1% aqueous formic acid at a flow rate of 0.25 mL/min. The detection of the analytes was performed on an electrospray ionization interface operating in positive‐ion and multiple reaction monitoring acquisition modes. The calibration curves of these analytes showed good linearity (<italic>r ></italic> 0.99) within the test ranges. The lower limit of quantification ranged from 0.4 to 20.1 ng/mL for the analytes. The intra‐ and interday precision and accuracy were all within ±15%, and the recoveries were higher than 80.0%. The validated method was successfully applied to a pharmacokinetic study of the nine flavonoids after administration of the <italic>Viscum coloratum</italic> extracts by intravenous injection.</p> </abstract>